JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB254507

人Flt-3 ligand ELISA试剂盒

Human Flt-3 ligand ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Flt-3 ligand ELISA Kit is a single-wash 90-min SimpleStep ELISA used to quantify Human Flt-3 ligand with a sensitivity of 2 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab327301

查看别名

Fms-related tyrosine kinase 3 ligand, Flt3 ligand, Flt3L, SL cytokine, FLT3LG

8 Images
ELISA - Human Flt-3 ligand ELISA Kit (AB254507)
  • ELISA

Supplier Data

ELISA - Human Flt-3 ligand ELISA Kit (AB254507)
Sandwich ELISA - Human Flt-3 ligand ELISA Kit (AB254507)
  • sELISA

Supplier Data

Sandwich ELISA - Human Flt-3 ligand ELISA Kit (AB254507)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Flt-3 Ligand concentration was determined to be 63.87 pg/mL with a range of 26.63 – 105.1 pg/mL.

Sandwich ELISA - Human Flt-3 ligand ELISA Kit (AB254507)
  • sELISA

Supplier Data

Sandwich ELISA - Human Flt-3 ligand ELISA Kit (AB254507)

Interpolated concentrations of native Flt-3 Ligand in human PBMC cell culture supernatant samples. Human PBMC were cultured in the presence (stimulated) or absence (unstimulated) of 1.5% PHA-M.

Human peripheral blood mononuclear cells (PBMC) were cultured in the presence (stimulated) or absence (unstimulated) of 1.5% PHA-M for 36 hours. The concentrations of Flt-3 Ligand were measured in duplicates, interpolated from the Flt-3 Ligand standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated PBMC supernatant 100% and unstimulated PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Flt-3 Ligand concentration was determined to be 22.72 pg/mL in neat PHA-M stimulated PBMC cell culture supernatant, 3.742 pg/mL in neat unstimulated PBMC cell culture supernatant, and undetectable in naive cell culture media (data not shown).

Sandwich ELISA - Human Flt-3 ligand ELISA Kit (AB254507)
  • sELISA

Supplier Data

Sandwich ELISA - Human Flt-3 ligand ELISA Kit (AB254507)

Interpolated concentrations of native Flt-3 Ligand in human serum and plasma samples.

The concentrations of Flt-3 Ligand were measured in duplicates, interpolated from the Flt-3 Ligand standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Flt-3 Ligand concentration was determined to be 65.35 pg/mL in neat serum, 57.01 pg/mL in neat plasma (citrate), 54.11 pg/mL in neat plasma (heparin), and 64.57 pg/mL in neat plasma (EDTA).

Sandwich ELISA - Human Flt-3 ligand ELISA Kit (AB254507)
  • sELISA

Lab

Sandwich ELISA - Human Flt-3 ligand ELISA Kit (AB254507)

Interpolated concentration of native Flt-3 Ligand was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% human serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Flt-3 ligand ELISA Kit (AB254507)
  • sELISA

Supplier Data

Sandwich ELISA - Human Flt-3 ligand ELISA Kit (AB254507)

Human Fit-3 Ligand standard curve comparison.

Standard Curve comparison between human Fit-3 Ligand SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human Flt-3 ligand ELISA Kit (AB254507)
  • sELISA

Lab

Sandwich ELISA - Human Flt-3 ligand ELISA Kit (AB254507)

Example of human Flt-3 Ligand standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Flt-3 ligand ELISA Kit (AB254507)
  • sELISA

Supplier Data

Sandwich ELISA - Human Flt-3 ligand ELISA Kit (AB254507)

Example of human Flt-3 Ligand standard curve in Sample Diluent NS.

The Flt-3 Ligand standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 2 pg/mL

范围

4.7 - 300 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

style
left-column

搭配使用产品

Assay Kits

AB327301

Human FLT-3 Ligand ELISA Kit, Chemiluminescent

SimpleStep Ignite™ ELISA|Recombinant

Sandwich ELISA - Human FLT-3 Ligand ELISA Kit, Chemiluminescent (AB327301)

  • 0
  • 0
Assay Kits

AB176109

Human SCF ELISA Kit

SimpleStep

ELISA - Human SCF ELISA Kit (AB176109)

  • 0
  • 0
Assay Kits

AB179886

Human MCP-1 ELISA Kit

Recombinant|SimpleStep

ELISA - Human MCP-1 ELISA Kit (AB179886)

  • 0
  • 0
Assay Kits

AB219632

Human Thrombopoietin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Thrombopoietin ELISA Kit (AB219632)

  • 0
  • 0
Assay Kits

AB270218

Human IL-7 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-7 ELISA Kit (AB270218)

  • 0
  • 0
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

Human Flt-3 ligand ELISA Kit ab254507 is a rapid single-wash 90-min Sandwich ELISA to measure Human Flt-3 ligand in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 2 pg/mL.

How the assay works

Human Flt-3 ligand SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Flt-3 ligand ELISA Kit ab254507 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human Flt-3 ligand ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Flt3 ligand/Flt3L antibody [EPR22288-52] - BSA and Azide free (Capture) ab259726
- Anti-Flt3 ligand/Flt3L antibody [EPR22288-201] - BSA and Azide free (Detector) ab259726

Flt-3 Ligand binds to and activates Flt-3. Flt-3 Ligand works synergistically with other CSFs and interleukins to stimulate growth and differentiation of early hematopoietic cells. The predominant form of Flt-3 Ligand is the biologically active transmembrane form which is proteolytically cleaved to generate a soluble form that is also biologically active. Alternative spliced secreted soluble variant of Flt-3 Ligand has also been identified.

style
left-column

精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" } ]
style
left-column

回收率

[ { "sample": "Cell culture supernatant", "range": "94 - 97 %", "average": "= 95" }, { "sample": "Serum", "range": "93 - 103 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "87 - 97 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "93 - 101 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "93 - 101 %", "average": "= 97" } ]
style
left-column

规格

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab254507-10x96Tests", "assetComponentDetails": [ { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB254507-CMP03" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB254507-CMP10" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB254507-CMP04" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB254507-CMP09" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB254507-CMP08" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB254507-CMP06" }, { "size":"1 x 6000 µL", "name":"10X Human Flt-3 ligand Capture Antibody", "number":"AB254507-CMP01" }, { "size":"2 x 10 Vial", "name":"Human Flt-3 ligand Lyophilized Recombinant Protein", "number":"AB254507-CMP07" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB254507-CMP05" }, { "size":"1 x 6000 µL", "name":"10X Human Flt-3 ligand Detector Antibody", "number":"AB254507-CMP02" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab254507-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB254507-CMP03" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB254507-CMP09" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB254507-CMP08" }, { "size":"1 x 600 µL", "name":"10X Human Flt-3 ligand Detector Antibody", "number":"AB254507-CMP02" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB254507-CMP04" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB254507-CMP06" }, { "size":"1 x 600 µL", "name":"10X Human Flt-3 ligand Capture Antibody", "number":"AB254507-CMP01" }, { "size":"2 x 1 Vial", "name":"Human Flt-3 ligand Lyophilized Recombinant Protein", "number":"AB254507-CMP07" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB254507-CMP05" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB254507-CMP10" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab254507-1x384Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human Flt-3 ligand Capture Antibody", "number":"AB254507-CMP01" }, { "size":"1 x 600 µL", "name":"10X Human Flt-3 ligand Detector Antibody", "number":"AB254507-CMP02" }, { "size":"1 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB254507-CMP03" }, { "size":"2 x 1 Vial", "name":"Human Flt-3 ligand Lyophilized Recombinant Protein", "number":"AB254507-CMP07" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB254507-CMP11" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB254507-CMP05" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB254507-CMP10" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB254507-CMP09" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB254507-CMP08" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB254507-CMP06" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab254507-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB254507-CMP09" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB254507-CMP11" }, { "size":"1 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB254507-CMP03" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB254507-CMP08" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB254507-CMP05" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB254507-CMP06" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB254507-CMP10" }, { "size":"2 x 1 Vial", "name":"Human Flt-3 ligand Lyophilized Recombinant Protein", "number":"AB254507-CMP07" }, { "size":"1 x 600 µL", "name":"10X Human Flt-3 ligand Capture Antibody", "number":"AB254507-CMP01" }, { "size":"1 x 600 µL", "name":"10X Human Flt-3 ligand Detector Antibody", "number":"AB254507-CMP02" } ] }, "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab254507-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB254507-CMP09" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB254507-CMP05" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB254507-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB254507-CMP06" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB254507-CMP08" }, { "size":"2 x 10 Vial", "name":"Human Flt-3 ligand Lyophilized Recombinant Protein", "number":"AB254507-CMP07" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB254507-CMP03" }, { "size":"1 x 6000 µL", "name":"10X Human Flt-3 ligand Capture Antibody", "number":"AB254507-CMP01" }, { "size":"1 x 6000 µL", "name":"10X Human Flt-3 ligand Detector Antibody", "number":"AB254507-CMP02" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB254507-CMP04" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab254507-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB254507-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB254507-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB254507-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB254507-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB254507-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB254507-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB254507-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Flt-3 ligand Capture Antibody", "number":"AB254507-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Flt-3 ligand Lyophilized Recombinant Protein", "number":"AB254507-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Flt-3 ligand Detector Antibody", "number":"AB254507-CMP02", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Flt3 ligand (Flt3L) also known as Flt3-L Flt3 ligand protein or flt-3 ligand protein is a small cytokine with a molecular mass of approximately 18 to 19 kDa. It acts as a growth factor by binding to the Flt3 receptor tyrosine kinase on the surface of hematopoietic progenitor cells. Flt3L is expressed in various tissues including the bone marrow where it plays a significant role in hematopoiesis. It is critical in dendritic cell and B cell development impacting the immune response mechanism.
Biological function summary

The Flt3 ligand plays an essential role in the immune system. It is a monomeric cytokine that induces proliferation of early lineage cells within the hematopoietic system. Flt3L does not function as part of a complex but acts independently to stimulate the proliferation differentiation and survival of hematopoietic cells. By interacting directly with its receptor it significantly influences the innate and adaptive immune systems through the maturation of dendritic and B cells.

Pathways

The Flt3 ligand is involved in hematopoietic signaling pathways primarily the PI3K-Akt signaling pathway. This pathway is essential for cell survival and growth where Flt3L aids in transmitting growth signals necessary for hematopoietic cells. Flt3L also interacts with proteins such as FLT3 receptor and influences the downstream signaling cascade affecting cell fate decisions in hematopoiesis and immune cell maturation.

Flt3 ligand is connected to leukemia particularly acute myeloid leukemia (AML) due to dysregulation of the FLT3 signaling pathway. Mutations in the FLT3 receptor can lead to uncontrolled cell proliferation contributing to tumorigenesis. Additionally Flt3L plays a role in autoimmune disorders by influencing immune cell development. It is closely connected with the FLT3 receptor and other tyrosine kinases which when mutated can exacerbate disease pathology through abnormal cell signaling and proliferation.
style
left-column

产品实验方案

style
left-column

靶点信息

Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. Required for the development of B cells, and dendritic cells (DCs).
See full target information FLT3LG
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com