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AB309188

人Filamin B ELISA试剂盒- Extracellular

Human Filamin B ELISA Kit - Extracellular

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Human Filamin B ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Human Filamin B with a sensitivity of 11.816 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

查看别名

FLN1L, FLN3, TABP, TAP, FLNB, Filamin-B, FLN-B, ABP-278, ABP-280 homolog, Actin-binding-like protein, Beta-filamin, Filamin homolog 1, Filamin-3, Thyroid autoantigen, Truncated actin-binding protein, Fh1, Truncated ABP

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Sandwich ELISA - Human Filamin B ELISA Kit - Extracellular (AB309188)
  • sELISA

Supplier Data

Sandwich ELISA - Human Filamin B ELISA Kit - Extracellular (AB309188)

Serum of eight individual healthy human donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Filamin B concentration was determined to be 1641.5 pg/mL with a range of 857.7 – 5105.4 pg/mL.

Sandwich ELISA - Human Filamin B ELISA Kit - Extracellular (AB309188)
  • sELISA

Supplier Data

Sandwich ELISA - Human Filamin B ELISA Kit - Extracellular (AB309188)

Interpolated concentration of native Filamin B was measured in duplicate at different sample concentrations. Undiluted samples are 50% serum, plasma (EDTA), plasma (citrate), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Filamin B ELISA Kit - Extracellular (AB309188)
  • sELISA

Supplier Data

Sandwich ELISA - Human Filamin B ELISA Kit - Extracellular (AB309188)

Example of human Filamin B standard curve. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 11.816 pg/mL

范围

39.063 - 2500 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human Filamin B SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Filamin B protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human Filamin B with 11.816 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.02" } ]
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回收率

[ { "sample": "Serum", "range": "78 - 94 %", "average": "= 85" }, { "sample": "EDTA Plasma", "range": "102 - 119 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "85 - 87 %", "average": "= 86" }, { "sample": "Heparin Plasma", "range": "89 - 103 %", "average": "= 94" } ]
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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro.
See full target information FLNB
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websiteProtocolBooklet
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