人Ficolin-2 ELISA试剂盒

产品详情

Human Ficolin-2 ELISA kit (ab275554) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Ficolin-2 protein in serum, plasma and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Ficolin-2 with 8.35 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Ficolin-2, initially identified as L-ficolin, in human is encoded by the FCN2 gene. Ficolin-2 belongs to the ficolin family of proteins and is characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolin-2 is predominantly expressed in the liver. Studies have reported it to have roles in carbohydrate binding and opsonic activities. Serum concentration of ficolin-2 varies considerably in healthy individuals. FCN2 promoter polymorphisms were associated with marked changes in the ficolin-2 serum concentration.