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AB241383

人Fibrinogen ELISA试剂盒- high sensitivity

Human Fibrinogen ELISA Kit - high sensitivity

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(12 Publications)

Human Fibrinogen ELISA Kit - high sensitivity is a single-wash 90-min SimpleStep ELISA used to quantify Human Fibrinogen - high sensitivity with a sensitivity of 29 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab326383

查看别名

Fibrinogen alpha chain, FGA

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ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • ELISA

Supplier Data

ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Lab

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Interpolated concentrations of human Fibrinogen in serum in 96-well vs. 384-well plates

Interpolated concentration of native Fibrinogen was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 670 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Lab

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Example of human Fibrinogen standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Percent reactivity against related fibrinogen proteins.

The following recombinant/native proteins were prepared at 50 ng/mL and assayed for cross-reactivity. Percent reactivity is determined by interpolating the protein concentrations from the standard curve and expressing the interpolated concentrations as a percentage of the protein concentration assayed at the same concentration.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Interpolated concentrations of native Fibrinogen in human urine, saliva, milk and cell culture supernatant samples.

The concentrations of Fibrinogen were measured in duplicates, interpolated from the Fibrinogen standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 1.7, saliva 1 : 133, milk 1 : 133 and HepG2 supernatant 1 : 500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibrinogen concentration was determined to be 5.93 ng/mL in urine, 934.5 ng/mL in saliva, 199.7 ng/mL in milk and 1.35 μg/mL in HepG2 supernatant.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native Fibrinogen was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native Fibrinogen was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Human Fibrinogen Standard Curve Comparison.

Standard Curve comparison between human Fibrinogen current (new) SimpleStep ELISA kit and original ELISA kit (ab208036). The current SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Example of human Fibrinogen standard curve in Sample Diluent NS.

The Fibrinogen standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Example of human Fibrinogen standard curve in Sample Diluent NS.

The Fibrinogen standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibrinogen concentration was determined to be 1,070 ng/mL with a range of 647 – 2,027 ng/mL.

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)
  • sELISA

Supplier Data

Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

Interpolated concentrations of native Fibrinogen in human serum and plasma samples.

The concentrations of Fibrinogen were measured in duplicates, interpolated from the Fibrinogen standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 670, plasma (citrate) 1 : 5x105, plasma (EDTA) 1 : 5x105, and plasma (heparin) 1 : 5x105. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibrinogen concentration was determined to be 3.45 μg/mL in serum, 2.86 mg/mL in plasma (citrate), 2.96 mg/mL in plasma (EDTA), and 2.64 mg/mL in plasma (heparin).

关键信息

检测方法

Colorimetric

样品类型

Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 29 pg/mL

范围

125 - 8000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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搭配使用产品

Assay Kits

AB326383

Human Fibrinogen ELISA Kit, Chemiluminescent

SimpleStep Ignite™ ELISA|Recombinant

Sandwich ELISA - Human Fibrinogen ELISA Kit, Chemiluminescent (AB326383)

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Assay Kits

AB189579

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1)

SimpleStep

ELISA - Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (AB189579)

  • 0
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Assay Kits

AB230933

Mouse Thrombin ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Thrombin ELISA Kit (AB230933)

  • 0
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Assay Kits

AB223864

Human Von Willebrand Factor ELISA Kit

Recombinant|SimpleStep

ELISA - Human Von Willebrand Factor ELISA Kit (AB223864)

  • 0
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human Fibrinogen ELISA Kit - high sensitivity ab241383 is a rapid single-wash 90-min ELISA kit to measure Human Fibrinogen in saliva, urine, cell culture supernatant, milk, plasma and serum. This SimpleStep ELISA® sensitivity is 29 pg/mL.

How the assay works
Human Fibrinogen SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity
This kit recognizes both native and recombinant human Fibrinogen protein in serum, plasma, urine, saliva, milk, and cell culture supernatant only. Cell and tissue extract samples have not been tested with this kit.

Human Fibrinogen ELISA Kit - high sensitivity ab241383 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human Fibrinogen ELISA Kit - high sensitivity ab241383
Human Fibrinogen ELISA Kit - high sensitivity ab241383 has been used to study fibrinogen function and the effects of a novel mutation on fibinogen synthesis, secretion, and polymerization(1), extracorporeal bioengineered liver grafts for treating acute liver failure(2), and CRSPR/Cas9 to upregulate expression of coagulation factors II, VII, IX, X, and fibrinogen(3).

References:
(1)Fei Chen et al. 2024, PMID: 38233949,
(2)Jeff J Ross et al. 2023, PMID: 37892931,
(3)Mark J Osborn et al. 2022, PMID: 35563479

Save your precious samples
Our 384-well Human Fibrinogen ELISA Kit - high sensitivity only requires a maxmum of 12.5µL

Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity ab256605
- Anti-Fibrinogen antibody [EPR19765-189] - BSA and Azide free (Capture) ab244636
- Anti-Fibrinogen antibody [EPR19765-63] - BSA and Azide free (Detector) ab244897

Related and recommended products
Human Fibrinogen ELISA Kit - high sensitivity ab241383 is commonly used to study fibrinogen levels in coagulation, fibrinolysis, and liver function.

Other ELISA kits to measure coagulation and liver health include:
- Human AST ELISA Kit (Aspartate Aminotransferase) ab263881
- Human PAI1 ELISA Kit (SERPINE1) ab269373
- Human Plasminogen ELISA Kit (PLG) ab108893

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精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]
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回收率

[ { "sample": "Cell culture supernatant", "range": "93 - 95 %", "average": "= 94" }, { "sample": "Milk", "range": "102 - 105 %", "average": "= 103" }, { "sample": "Serum", "range": "90 - 94 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "91 - 94 %", "average": "= 93" }, { "sample": "Urine", "range": "99 - 100 %", "average": "= 100" }, { "sample": "Saliva", "range": "98 - 104 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": null, "average": "= 95" }, { "sample": "Citrate plasma", "range": "91 - 96 %", "average": "= 94" } ]
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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.
See full target information FGA
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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:13013 PubMed40234551

2025

Serum fibrinogen is not elevated in patients with myasthenia gravis.

Applications

Unspecified application

Species

Unspecified reactive species

Taylor A Bauman,Sean M Lee,Vern C Juel,Yingkai Li,Karli Gilbert,Jiaxin Chen,Henry J Kaminski,Linda L Kusner

Physiological reports 12:e70147 PubMed39667826

2024

Eccentric exercise-induced muscle damage and inflammation in conjunction with high-altitude decompression in adults.

Applications

Unspecified application

Species

Unspecified reactive species

Frode Gottschalk,Mikael Gennser,Mattias Günther,Ola Eiken,Antonis Elia

Biomedicines 12: PubMed38927479

2024

First Application of a Mixed Porcine-Human Repopulated Bioengineered Liver in a Preclinical Model of Post-Resection Liver Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Philipp Felgendreff,Seyed Mohammad Hosseiniasl,Anna Minshew,Bruce P Amiot,Silvana Wilken,Boyukkhanim Ahmadzada,Robert C Huebert,Nidhi Jalan Sakrikar,Noah G Engles,Peggy Halsten,Kendra Mariakis,John Barry,Shawn Riesgraf,Chris Fecteau,Jeffrey J Ross,Scott L Nyberg

AMB Express 14:66 PubMed38842656

2024

Microbial reduction of prebagged human plasma using 405 nm light and its effects on coagulation factors.

Applications

Unspecified application

Species

Unspecified reactive species

Caitlin F Stewart,Preston McGoldrick,John G Anderson,Scott J MacGregor,Chintamani D Atreya,Michelle Maclean

Nature communications 15:2704 PubMed38538626

2024

Fibrinolytic-deficiencies predispose hosts to septicemia from a catheter-associated UTI.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan J Molina,Kurt N Kohler,Christopher Gager,Marissa J Andersen,Ellsa Wongso,Elizabeth R Lucas,Andrew Paik,Wei Xu,Deborah L Donahue,Karla Bergeron,Aleksandra Klim,Michael G Caparon,Scott J Hultgren,Alana Desai,Victoria A Ploplis,Matthew J Flick,Francis J Castellino,Ana L Flores-Mireles

Toxicological sciences : an official journal of the Society of Toxicology 199:227-245 PubMed38335931

2024

Development of a human liver microphysiological coculture system for higher throughput chemical safety assessment.

Applications

Unspecified application

Species

Unspecified reactive species

Blanche C Ip,Samantha J Madnick,Sophia Zheng,Tessa C A van Tongeren,Susan J Hall,Hui Li,Suzanne Martin,Sandrine Spriggs,Paul Carmichael,Wei Chen,David Ames,Lori A Breitweiser,Heather E Pence,Andrew J Bowling,Kamin J Johnson,Richard Cubberley,Jeffrey R Morgan,Kim Boekelheide

Hereditas 161:4 PubMed38233949

2024

A novel missense mutation (FGG c.1168G > T) in the gamma chain of fibrinogen causing congenital hypodysfibrinogenemia with bleeding phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Nuo Xu,Liping Zheng,Zhehao Dai,Jun Zhu,Peng Xie,Shun Yang,Fei Chen

Bioengineering (Basel, Switzerland) 10: PubMed37892931

2023

Manufacturing and Functional Characterization of Bioengineered Liver Grafts for Extracorporeal Liver Assistance in Acute Liver Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria L Nelson,Aron R Stumbras,R Noelle Palumbo,Shawn A Riesgraf,Marie S Balboa,Zachary A Hannah,Isaac J Bergstrom,Christopher J Fecteau,John R Lake,John J Barry,Jeff J Ross

International journal of molecular sciences 24: PubMed37239814

2023

Engineering Human Cells Expressing CRISPR/Cas9-Synergistic Activation Mediators for Recombinant Protein Production.

Applications

Unspecified application

Species

Unspecified reactive species

Colby J Feser,James M Williams,Daniel T Lammers,Jason R Bingham,Matthew J Eckert,Jakub Tolar,Mark J Osborn

International journal of molecular sciences 23: PubMed36142345

2022

SARS-CoV-2 Spike Proteins and Cell-Cell Communication Inhibits TFPI and Induces Thrombogenic Factors in Human Lung Microvascular Endothelial Cells and Neutrophils: Implications for COVID-19 Coagulopathy Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Biju Bhargavan,Georgette D Kanmogne
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