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人Fetuin A ELISA试剂盒(AHSG) (ab108855)

Submit a review Q&A (1)References (7)
Typical Standard Curve

    Key features and details

    • Sensitivity: 1.4 ng/ml
    • Range: 3.125 ng/ml - 200 ng/ml
    • Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    概述

    • 产品名称

      人Fetuin A ELISA试剂盒(AHSG)
      参阅全部 AHSG 试剂盒

    • 检测方法

      Colorimetric

    • 精确度

      批次内
      样品 n Mean SD CV%
      Overall 3.6%
      批次间
      样品 n Mean SD CV%
      Overall 8.7%

    • 样品类型

      Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid

    • 检测类型

      Sandwich (quantitative)

    • 灵敏度

      1.4 ng/ml

    • 范围

      3.125 ng/ml - 200 ng/ml

    • 回收率

      98 %

    • 检测时间

      4h 00m

    • 实验步骤

      Multiple steps standard assay

    • 种属反应性

      与反应: Human

    • 产品概述

      Abcam’s Fetuin A (AHSG) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fetuin A concentrations in Human plasma, serum, urine, milk and cell culture supernatants.


      A Fetuin A specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fetuin A specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fetuin A captured in plate.


      Get higher sensitivity in only 90 minutes with Human Fetuin A ELISA Kit (AHSG) (ab185989) from our SimpleStep ELISA® range.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • 经测试应用

      适用于: Sandwich ELISAmore details

    • 平台

      Microplate

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab108855于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Sandwich ELISA
    Use at an assay dependent concentration.
    说明
    Sandwich ELISA
    Use at an assay dependent concentration.

    图片

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108855.

    文献 (7)

    发表研究结果有使用 ab108855？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab108855 被引用在 7 文献中.

    • Lin X  et al. Plasma Exosomes Derived From Patients With End-Stage Renal Disease and Renal Transplant Recipients Have Different Effects on Vascular Calcification. Front Cell Dev Biol 8:618228 (2020). PubMed: 33585452
    • Sieckmann T  et al. Longitudinal studies of putative growth hormone (GH) biomarkers and hematological and steroidal parameters in relation to 2 weeks administration of human recombinant GH. Drug Test Anal 12:711-719 (2020). PubMed: 32115881
    • Riedelová Z  et al. SPR biosensor for quantification of fetuin-A as a promising multibiomarker. Physiol Res 67:S367-S375 (2018). PubMed: 30379557
    • Niu R  et al. iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis. PLoS One 12:e0170741 (2017). ELISA ; Human . PubMed: 28122020
    • Abdullah MI  et al. Tissue and serum samples of patients with papillary thyroid cancer with and without benign background demonstrate different altered expression of proteins. PeerJ 4:e2450 (2016). Sandwich ELISA ; Human . PubMed: 27672505
    • Májek P  et al. Plasma proteome changes associated with refractory anemia and refractory anemia with ringed sideroblasts in patients with myelodysplastic syndrome. Proteome Sci 11:14 (2013). PubMed: 23566303
    • Kuakarn S  et al. Serum proteins in chronic hepatitis B patients treated with peginterferon alfa-2b. World J Gastroenterol 19:5067-75 (2013). Sandwich ELISA ; Human . PubMed: 23964140

    客户评价及客户问答

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    Question

    Does the Alpha 2 HS Glycoprotein Human ELISA kit (ab108855) cross react
    with bovine? Would Alpha 2 HS Glycoprotein from FBS interfere with the
    assay?

    Read More
    Answer

    Thank you for your patience, I apologize for the delay in my reply. The lab has confirmed that this kit will not cross-react with bovine alpha 2 HS Glycoprotein, and that FBS will not interfere with your results. I hope this helps, please let me know if you need any additional information or assistance.

    Read More

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