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AB324325

Human Factor XI ELISA Kit

Human Factor XI ELISA Kit

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Human Factor XI ELISA Kit is a Sandwich ELISA for the measurement of Human Factor XI in Human in Biofluids, Cell Culture Media samples.

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Coagulation factor XI, FXI, Plasma thromboplastin antecedent, PTA, F11

6 Images
Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)

Example of human Factor XI standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)

Interpolated concentration of native Factor XI was measured in duplicate at different sample concentrations. Undiluted samples are 50% HepG2 cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)

Example of human Factor XI standard curve and human Factor XIa NISBC standard (13/100) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)

Interpolated concentration of native Factor XI was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 2,000 human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)

Example of human Factor XI standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor XI ELISA Kit (AB324325)

Interpolated concentration of native Factor XI was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 2,000 human serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

关键信息

检测方法

Colorimetric

样品类型

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

反应种属

Human

检测类型

Sandwich

灵敏度

= 45.115 pg/mL

范围

156.25 - 10000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human Factor XI SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Factor XI protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human Factor XI with 45.115 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.9" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.3" } ]
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回收率

[ { "sample": "Serum", "range": "89 - 94 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "99 - 112 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "86 - 91 %", "average": "= 88" }, { "sample": "Heparin Plasma", "range": "98 - 112 %", "average": "= 106" }, { "sample": "Cell culture supernatant", "range": "84 - 85 %", "average": "= 85" } ]
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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
See full target information F11
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websiteProtocolBooklet
en
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