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人Factor XI ELISA试剂盒(ab108834)

Submit a review Q&A (3)References (1)
Typical Standard Curve

    Key features and details

    • Sensitivity: 0.75 ng/ml
    • Range: 1.563 ng/ml - 50 ng/ml
    • Sample type: Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    概述

    • 产品名称

      人Factor XI ELISA试剂盒
      参阅全部 FXI 试剂盒

    • 检测方法

      Colorimetric

    • 精确度

      批次内
      样品 n Mean SD CV%
      Overall 3.1%
      批次间
      样品 n Mean SD CV%
      Overall 9.9%

    • 样品类型

      Serum, Plasma

    • 检测类型

      Sandwich (quantitative)

    • 灵敏度

      >= 0.75 ng/ml

    • 范围

      1.563 ng/ml - 50 ng/ml

    • 回收率

      91 %

    • 检测时间

      4h 00m

    • 实验步骤

      Multiple steps standard assay

    • 种属反应性

      与反应: Human

    • 产品概述

      Abcam’s Factor XI Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor XI levels in human plasma and serum samples.


      A Factor XI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XI captured in plate.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • 经测试应用

      适用于: Sandwich ELISAmore details

    • 平台

      Microplate

    性能

    • 存放说明

      Store at -20°C. Please refer to protocols.
    • 组件 1 x 96 tests
      100X Streptavidin-Peroxidase Conjugate 1 x 80µl
      10X Diluent M Concentrate 1 x 30ml
      20X Wash Buffer Concentrate 2 x 30ml
      50X Biotinylated Human Factor XI Antibody 1 x 120µl
      Chromogen Substrate 1 x 7ml
      Factor XI Microplate (12 x 8 well strips) 1 unit
      Factor XI Standard 1 vial
      Sealing Tapes 3 units
      Stop Solution 1 x 11ml

    • 研究领域

    • 功能

      Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.

    • 组织特异性

      Isoform 2 is produced by platelets and megakaryocytes but absent from other blood cells.

    • 疾病相关

      Defects in F11 are the cause of factor XI deficiency (FA11D) [MIM:612416]; also known as plasma thromboplastin antecedent deficiency or Rosenthal syndrome. It is a hemorrhagic disease characterized by reduced levels and activity of factor XI resulting in moderate bleeding symptoms, usually occurring after trauma or surgery. Patients usually do not present spontaneous bleeding but women can present with menorrhagia. Hemorrhages are usually moderate.

    • 序列相似性

      Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
      Contains 4 apple domains.
      Contains 1 peptidase S1 domain.

    • 翻译后修饰

      Activated by factor XIIa (or XII), which cleaves each polypeptide after Arg-387 into the light chain, which contains the active site, and the heavy chain, which associates with high molecular weight (HMW) kininogen.

    • 细胞定位

      Secreted.
    • Information by UniProt

    • 别名

      • Coagulation factor XI
      • Coagulation factor XIa light chain
      • F11
      • FA11_HUMAN
      • Factor XI
      • FXI
      • MGC141891
      • Plasma thromboplastin antecedent
      • PTA
      see all

    • 数据库链接

      应用

      The Abpromise guarantee

      Abpromise™承诺保证使用ab108834于以下的经测试应用

      “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

      应用 Ab评论 说明
      Sandwich ELISA
      Use at an assay dependent concentration.
      说明
      Sandwich ELISA
      Use at an assay dependent concentration.

      图片

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab108834.

      文献 (1)

      发表研究结果有使用 ab108834？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab108834 被引用在 1 文献中.

      • Wu YW  et al. Dedicated removal of immunoglobulin (Ig)A, IgM, and Factor (F)XI/activated FXI from human plasma IgG. Transfusion 54:169-78 (2014). PubMed: 23701287

      客户评价及客户问答

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      1-3 of 3 Abreviews or Q&A

      Question

      Dear Josephine



      Thanks for your kindly reply, this customer also wants to know the retention period of the “Factor XI standard”. Could you please help this customer to solve the problem?



      Thanks for your kindly help

      Best regards,

      Ada




      呂小姐 (Ada Lu)
      Technical Support
      Interlab Co., Ltd (卓昇有限公司)
      Phone: +886-2-27367100
      Fax: +886-2-27359807



      -----Original Message-----
      From: mailto:hk.technical@abcam.com [mailto:hk.technical@abcam.com]
      Sent: Wednesday, September 26, 2012 10:15 AM
      To: mailto:technical@interlab.com.tw
      Subject: Reply from Abcam to your enquiry regarding ab108834 [CCE4185631]

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      https://www.abcam.com/index.html?utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Header









      Dear Technical Service



      Thank you for contacting us.

      The plate can last for at least one year from the date received the kit as long as the pouch un-open. If the pouch has been opened, please keep it in the desicator if you have one. Keep dry is very important. If you keep the open plate dry, it can last at least one to two weeks. It depends on your storage opened plate, sometimes can last for a few month. If you put the opened plated in a desicator and has sealed inside the desicator, it should last for at least one month. It is hard to give a definite time after the plate has opened.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews


      Help us improve our service.
      https://www.abcam.com/index.html?pageConfig=technicalSurvey&intCCEID=4185631


      Best regards,
      Josephine

      Josephine Au, PhD
      Scientific Support Supervisor
      Abcam (Hong Kong) Ltd.
      www.abcam.com


      Your original inquiry to Abcam:


      Product code: 108834



      Inquiry: Dear technical support team: This customer raised an enquire about ab108834 (Factor XI Human ELISA Kit), he wants to know the retention period of the “Factor XI Microplate (12 strips of 8 wells)”, could you please help this customer to solve the problem? Thanks for your kindly help Best regards, Ada








      Abcam Customer Services and Scientific Support Team
      https://www.abcam.com/index.html?pageconfig=technical&utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Body

      [CCE4185631]










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      Answer

      Thank you for contacting us.

      1. At the power form, it should be good for around 1 year.
      2. After dissolve in buffer, the standard need to be aliquot and store at -80 degree if possible. If store at -80, the standard can be good for at least one month. Our internal data suggest it may still be usable store at -80 after three months.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Question

      Product code: 108834

      Inquiry: Dear technical support team: This customer raised an enquire about ab108834 (Factor XI Human ELISA Kit), he wants to know the retention period of the “Factor XI Microplate (12 strips of 8 wells)”, could you please help this customer to solve the problem? Thanks for your kindly help Best regards, Ada

      Read More
      Answer

      Thank you for contacting us.

      The plate can last for at least one year from the date received the kit as long as the pouch un-open. If the pouch has been opened, please keep it in the desicator if you have one. Keep dry is very important. If you keep the open plate dry, it can last at least one to two weeks. It depends on your storage opened plate, sometimes can last for a few month. If you put the opened plated in a desicator and has sealed inside the desicator, it should last for at least one month. It is hard to give a definite time after the plate has opened.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Question

      Merci de votre réponse. Toutefois elle me donne une information partielle. Ma question était " combien de dosage de facteur XI différés, dans le temps, peut on faire avec un seul Kit".? Aujourd'hui je sais que le kit n'est pas utilisable plus que deux mois. Mais est ce que faire un dosage consomme tout le kit? Si non combien de dosages peut-on faire - autrement dit combien de patients peut-on doser dans une période de deux mois?    

      Read More
      Answer

      Comme indiqué dans le protocol du kit ELISA facteur XI ab108834 (https://www.abcam.com/ps/products/108/ab108834/documents/ab108834%20Factor%20XI%20Human%20ELISA_v3%20%28Website%29.pdf), il est conseillé d'effectuer 2 ou 3 séries de 7 points de standards (entre 0 et 25ng/ml). Ce qui vous laisse la possibilité de mesurer sur la plaque 96 puits entre 75 et 82 échantillons. Donc pour une utilisation unique, si vous désirez répéter par exemple 3 fois la mesure pour un même patient et avoir les concentrations standard mesurées également 3 fois, le nombre de patients pouvant être étudiés avec un kit est de 25. Détail du calcul : [96-(3x7)]/3 = 25. J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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