人Factor XI ELISA试剂盒

产品详情

Human Factor XI ELISA Kit is a sandwich ELISA kit to measure Human Factor XI in plasma, serum with a sensitivity of 0.75 ng/mL.

How the assay works

A Factor XI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XI captured in plate.

Assay Specificity

No significant cross-reactivity observed with human factor I (fibrinogen), factor II (prothrombin), factor III (tissue factor), factor V, factor VII, factor VIII, factor IX, factor X, factor XII, and factor XIII, factor XIV (protein C), and VWF proteins

Human Factor XI ELISA Kit ab108834 protocol summary:

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared StreptavidinPeroxidase Conjugate. Incubate at room temperature.
4. Add Chromogen Substrate to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read immediately.