人Factor X ELISA试剂盒(ab108832)
Key features and details
- Sensitivity: 0.16 ng/ml
- Range: 0.781 ng/ml - 50 ng/ml
- Sample type: Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Factor X ELISA试剂盒
参阅全部 Factor X 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5.3% 批次间 样品 n Mean SD CV% Overall 10.2% -
样品类型
Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
检测类型
Sandwich (quantitative) -
灵敏度
>= 0.16 ng/ml -
范围
0.781 ng/ml - 50 ng/ml -
回收率
98 %
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检测时间
4h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Human Factor Xin vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor X concentrations in plasma, serum, milk, urine, saliva, and cerebrospinal fluid.
A Factor X specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor X specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor X captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Biotinylated Antibody 1 x 60µl 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml Chromogen Substrate 1 x 7ml Factor X Microplate (12 x 8 well strips) 1 unit Factor X Standard 1 x 65ng Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. -
组织特异性
Plasma; synthesized in the liver. -
疾病相关
Defects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis. -
序列相似性
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
翻译后修饰
The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
细胞定位
Secreted. - Information by UniProt
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别名
- Activated factor Xa heavy chain
- Coagulation factor X
- F10
see all -
数据库链接
- Entrez Gene: 2159 Human
- Omim: 227600 Human
- SwissProt: P00742 Human
- Unigene: 361463 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108832于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab108832
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ELISA: Factor X Human ELISA kit (ab108832)Standard curve with background signal subtracted (duplicates; +/- SD).
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ELISA: Factor X Human ELISA kit (ab108832)Factor X measured in undiluted culture supernatants with MCF7 levels below level of detection (duplicates +/- SD).
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ELISA: Factor X Human ELISA kit (ab108832)Factor X measured in biological fluids (duplicates +/- SD). Serum and plasma were tested at 1/300-1/3000; other samples at 1/1-1/30.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab108832 被引用在 3 文献中.
- Feser CJ et al. Engineering Human Cells Expressing CRISPR/Cas9-Synergistic Activation Mediators for Recombinant Protein Production. Int J Mol Sci 24:N/A (2023). PubMed: 37239814
- Feser CJ et al. Engineering CRISPR/Cas9 for Multiplexed Recombinant Coagulation Factor Production. Int J Mol Sci 23:N/A (2022). PubMed: 35563479
- Cohen CT et al. Production and control of coagulation proteins for factor X activation in human endothelial cells and fibroblasts. Sci Rep 10:2005 (2020). PubMed: 32029851