AB108832

人Factor X ELISA试剂盒

Name: 人Factor X ELISA试剂盒 (ab108832)| Abcam中文官网
Brand: Abcam Limited
SKU: AB108832
Price: 7527 CNY
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Human Factor X ELISA Kit

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(3 Publications)

Human Factor X ELISA Kit is a sandwich ELISA designed to quantify Human Factor X with a sensitivity of 0.16 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Wide dynamic range - quantifies 0.781 - 50 ng/mL

查看别名

Coagulation factor X, Stuart factor, Stuart-Prower factor, F10

4 Images

ELISA - Human Factor X ELISA Kit (AB108832)

ELISA

Lab

ELISA - Human Factor X ELISA Kit (AB108832)

Standard curve with background signal subtracted (duplicates; +/- SD).

ELISA

Lab

ELISA - Human Factor X ELISA Kit (AB108832)

Factor X measured in biological fluids (duplicates +/- SD). Serum and plasma were tested at 1/300-1/3000; other samples at 1/1-1/30.

ELISA

Lab

ELISA - Human Factor X ELISA Kit (AB108832)

Factor X measured in undiluted culture supernatants with MCF7 levels below level of detection (duplicates +/- SD).

sELISA

Supplier Data

Sandwich ELISA - Human Factor X ELISA Kit (AB108832)

Representative Standard Curve using ab108832

关键信息

检测方法

Colorimetric

样品类型

Cerebral Spinal Fluid, Saliva, Urine, Plasma, Milk, Serum

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

>= 0.16 ng/mL

范围

0.781 - 50 ng/mL

检测时间

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human Factor X ELISA Kit ab108832 is a sandwich ELISA to measure Human Factor X in serum, plasma, cerebral spinal fluid (CSF), saliva, urine, milk with a sensitivity of 0.16 ng/ml.

How the assay works

A Factor X specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor X specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor X captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Factor X ELISA Kit ab108832 protocol summary

1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.3" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 10.2" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108832-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108832-CMP06", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108832-CMP07", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108832-CMP08", "productcode":"" }, { "size":"1 x 60 µL", "name":"100X Biotinylated Antibody", "number":"AB108832-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Factor X Microplate (12 x 8 well strips)", "number":"AB108832-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108832-CMP02", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108832-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108832-CMP05", "productcode":"" }, { "size":"1 x 65 ng", "name":"Factor X Standard", "number":"AB108832-CMP04", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

Please refer to protocols

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Factor X also known as Stuart-Prower Factor is a critical component in the coagulation cascade. It has a molecular weight of approximately 59 kDa and is expressed mainly in the liver. This serine protease plays an important role in the conversion of prothrombin to thrombin an essential step in blood clot formation. Factor X circulates in the plasma as an inactive zymogen and becomes activated to its enzyme form factor Xa in response to specific physiological signals.

Biological function summary

Factor X contributes significantly to the coagulation process by activating prothrombin into thrombin. It is not part of a larger complex but functions closely with other components of the coagulation cascade to maintain hemostasis. Factor X interacts with calcium ions and phospholipids on the surface of platelets enhancing its enzymatic activity. This activation ensures proper regulation of blood clotting preventing excessive bleeding or uncontrolled clot formation.

Pathways

Factor X is central to both the intrinsic and extrinsic coagulation pathways. It interacts with proteins such as factor VIIa in the extrinsic pathway and factor IXa in the intrinsic pathway both pathways leading to the activation of factor Xa. In turn factor Xa is key in the common pathway which combines inputs from the intrinsic and extrinsic pathways to produce thrombin essential for fibrin clot formation.

Factor X is associated with bleeding disorders such as hemophilia and liver disease. Deficiencies or dysfunctions in factor X result in inadequate clot formation leading to prolonged bleeding episodes. Furthermore increased factor X activity as in cases of thrombophilia can raise the risk of abnormal blood clots. Factor X works alongside proteins like factor V with both deficiencies playing roles in hemophilia. Monitoring factor X activity is important in diagnosing and managing these coagulation-related conditions.

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产品实验方案

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靶点信息

Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting (PubMed : 22409427). Factor Xa activates pro-inflammatory signaling pathways in a protease-activated receptor (PAR)-dependent manner (PubMed : 24041930, PubMed : 30568593, PubMed : 34831181, PubMed : 18202198). Up-regulates expression of protease-activated receptors (PARs) F2R, F2RL1 and F2RL2 in dermal microvascular endothelial cells (PubMed : 35738824). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, in cardiac fibroblasts and umbilical vein endothelial cells in PAR-1/F2R-dependent manner (PubMed : 30568593, PubMed : 34831181). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2, IL6, TNF-alpha/TNF, IL-1beta/IL1B, IL8/CXCL8 and IL18, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Induces expression of adhesion molecules, such as ICAM1, VCAM1 and SELE, in endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824, PubMed : 9780208). Increases expression of phosphorylated ERK1/2 in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Triggers activation of the transcription factor NF-kappa-B in dermal microvascular endothelial cells and atrial tissues (PubMed : 24041930, PubMed : 35738824). Activates pro-inflammatory and pro-fibrotic responses in dermal fibroblasts and enhances wound healing probably via PAR-2/F2RL1-dependent mechanism (PubMed : 18202198). Activates barrier protective signaling responses in endothelial cells in PAR-2/F2RL1-dependent manner; the activity depends on the cleavage of PAR-2/F2RL1 by factor Xa (PubMed : 22409427). Up-regulates expression of plasminogen activator inhibitor 1 (SERPINE1) in atrial tissues (PubMed : 24041930).

See full target information F10

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37239814

2023

Engineering Human Cells Expressing CRISPR/Cas9-Synergistic Activation Mediators for Recombinant Protein Production.

Applications

Unspecified application

Species

Unspecified reactive species

Colby J Feser,James M Williams,Daniel T Lammers,Jason R Bingham,Matthew J Eckert,Jakub Tolar,Mark J Osborn

International journal of molecular sciences 23: PubMed35563479

2022

Engineering CRISPR/Cas9 for Multiplexed Recombinant Coagulation Factor Production.

Applications

Unspecified application

Species

Unspecified reactive species

Colby J Feser,Christopher J Lees,Daniel T Lammers,Megan J Riddle,Jason R Bingham,Matthew J Eckert,Jakub Tolar,Mark J Osborn

Scientific reports 10:2005 PubMed32029851

2020

Production and control of coagulation proteins for factor X activation in human endothelial cells and fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Clay T Cohen,Nancy A Turner,Joel L Moake

View all publications

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websiteProtocolBooklet

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人Factor X ELISA试剂盒

Human Factor X ELISA Kit

ELISA - Human Factor X ELISA Kit (AB108832)

ELISA - Human Factor X ELISA Kit (AB108832)

ELISA - Human Factor X ELISA Kit (AB108832)

Sandwich ELISA - Human Factor X ELISA Kit (AB108832)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台