人Factor VII ELISA试剂盒
Human Factor VII ELISA Kit
- Recombinant
- SimpleStep
- 了解详情
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(1 Publication)
Human Factor VII ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Factor VII with a sensitivity of 0.16 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
查看别名
Coagulation factor VII, Proconvertin, Serum prothrombin conversion accelerator, SPCA, F7
- sELISA
Lab
Sandwich ELISA - Human Factor VII ELISA Kit (AB190810)
Representative image of Human Factor VII ELISA Kit (ab190810)
Components shown from left to right :
- Sample Diluent NS (ab193972)
- 10X Human Factor VII Capture Antibody
- 10X Human Factor VII Detector Antibody
- Human Factor VII Lyophilized Purified Protein (2 vials)
- 50X Cell Extraction Enhancer Solution (ab193971)
- Stop Solution
- TMB development solution
- Antibody Diluent 4BI
- 10X Wash Buffer PT (ab206977)
SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.
Plate Seals - shown underneath bottles / vials.
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.
- sELISA
Supplier Data
Sandwich ELISA - Human Factor VII ELISA Kit (AB190810)
Serum from eight individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FVII concentration was determined to be 536 ng/mL with a range of 316 – 800 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human Factor VII ELISA Kit (AB190810)
Interpolated concentrations of native FVII in human serum and plasma samples.
The concentrations of FVII were measured in duplicates, interpolated from the FVII standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2.5%, plasma (EDTA) 2.5%, plasma (citrate) 5% and serum (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FVII concentration was determined to be 513 ng/mL in serum, 642 ng/mL in plasma (EDTA), 368 ng/mL in plasma (citrate), and 517 ng/mL in plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human Factor VII ELISA Kit (AB190810)
Example of human FVII standard curve in Sample Diluent NS + Enhancer.
The FVII standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
反应性数据
产品详情
As of July 17, 2020, Human Factor VII ELISA kit has been re-developed with new recombinant monoclonal antibodies to provide improved performance and consistency. Please note the protocol has also been updated.
Human Factor VII ELISA Kit (ab190810) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Factor VII protein in cit plasma, hep plasma, serum, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Factor VII with 292 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Factor VII is a plasma serine protease involved in the coagulation cascade. Processed in the liver, it circulates in the bloodstream as a zymogen. During coagulation it is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin. Factor VIIa can then convert factor IX to IXa in the presence of tissue factor and calcium.
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性能和储存信息
运输条件
推荐的短期储存条件
推荐的长期储存条件
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Factor 7a indirectly activates further components in the blood coagulation cascade. Upon activation factor 7a becomes a part of the tissue factor (TF) complex which includes tissue factor expressed on subendothelial cells. The complex triggers the activation of factor IX and factor X propagating the coagulation cascade downstream. This series of activations amplifies the signal for clot formation highlighting factor VII's integral role in initiating coagulation.
Pathways
Factor VII is primarily involved in the extrinsic pathway of the coagulation cascade. This pathway is initiated when factor 7a forms a complex with tissue factor a protein that becomes exposed at injury sites. Factor VII is also indirectly linked to the common coagulation pathway through activation of factor X. The interaction between these pathways ensures efficient and rapid clot formation. Additionally factor IX also interacts with factor VII in the intricate network of coagulation processes that sustain hemostasis.
靶点信息
文献 (1)
Recent publications for all applications. Explore the full list and refine your search
Journal of gastroenterology and hepatology 34:1851-1859 PubMed30884543
2019
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Unspecified application
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Arabidopsis suecica
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