人FABP5 ELISA试剂盒(ab272474)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.14 ng/ml
- Range: 0.31 ng/ml - 20 ng/ml
- Sample type: EDTA Plasma, Hep Plasma, Saliva, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人FABP5 ELISA试剂盒
参阅全部 FABP5 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 8 2.8% 批次间 样品 n Mean SD CV% Serum 3 6% -
样品类型
Saliva, Urine, Serum, Tissue Extracts, Hep Plasma, EDTA Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
0.14 ng/ml -
范围
0.31 ng/ml - 20 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Saliva 99 96% - 102% Urine 83 81% - 83% Serum 105 94% - 116% Hep Plasma 93 81% - 101% EDTA Plasma 83 81% - 84% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human FABP5 ELISA kit (ab272474) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human FABP5 protein in serum, plasma, urine, saliva and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FABP5 with 0.14 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Fatty acid-binding protein 5 (FABP5), also known as E-FABP for Epidermal-type fatty acid-binding protein, is a member of the fatty acid binding proteins and is encoded by the FABP5 gene. FABP5 is an a 134- amino acid containing protein that can be found either as a monomer or a homodimer. FABP5 mainly serves as an intracellular carrier for long-chain fatty acids and related lipids, which regulates metabolism. FABP5 can also transport specific fatty acids, such as retinoic acid and endocannabinoid, from the cytosol to the nucleus, where they activate nuclear receptors.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human FABP5 Capture Antibody 1 x 600µl 10X Human FABP5 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml Antibody Diluent CPI2 1 x 6ml Human FABP5 Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation. -
组织特异性
Keratinocytes; highly expressed in psoriatic skin. -
序列相似性
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
结构域
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
细胞定位
Cytoplasm. - Information by UniProt
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别名
- CFABP
- Cutaneous fatty acid binding protein
- DA11
see all -
数据库链接
- Entrez Gene: 2171 Human
- Omim: 605168 Human
- SwissProt: Q01469 Human
- Unigene: 408061 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272474于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human FABP5 standard curve in Sample Diluent NS.The FABP5 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native FABP5 in human saliva.The concentrations of FABP5 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 1.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 766 ng/mL in saliva.
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Interpolated concentrations of native FABP5 in human urine.The concentrations of FABP5 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 8.2 ng/mL in urine.
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Serum from eight individual healthy FABP5 male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP5 concentration was determined to be 3.4 ng/mL with a range of 0.8 – 11.2 ng/mL.
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Sandwich ELISA - Human FABP5 ELISA Kit (ab272474)To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
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