人FABP2 ELISA试剂盒(ab234566)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.7 pg/ml
- Range: 12.5 pg/ml - 800 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
-
产品名称
人FABP2 ELISA试剂盒
参阅全部 I-FABP 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 9 1.8% 批次间 样品 n Mean SD CV% Serum 3 3.3% -
样品类型
Cell culture supernatant, Urine, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
1.7 pg/ml -
范围
12.5 pg/ml - 800 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Urine 83 82% - 85% Serum 119 111% - 125% Cell culture extracts 95 92% - 97% Cell culture media 100 98% - 102% Hep Plasma 108 103% - 110% EDTA Plasma 115 109% - 120% Cit plasma 113 105% - 118% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human
不与反应: Cow -
产品概述
Human FABP2 ELISA Kit (ab234566) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FABP2 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FABP2 with 1.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
说明
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human FABP2 Capture Antibody 1 x 600µl 10X Human FABP2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human FABP2 Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
-
功能
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor. -
组织特异性
Expressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum. -
序列相似性
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
结构域
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
细胞定位
Cytoplasm. - Information by UniProt
-
别名
- FABP 2
- FABP2
- FABPI
see all -
数据库链接
- Entrez Gene: 2169 Human
- Omim: 134640 Human
- SwissProt: P12104 Human
- Unigene: 282265 Human
相关产品
-
Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab234566于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
-
Example of human FABP2 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
-
Example of human FABP2 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native FABP2 in human serum, plasma, and LoVo cell extract samples.The concentrations of FABP2 were measured in duplicates, interpolated from the FABP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (heparin) 50%, plasma (EDTA) 25%, plasma (citrate) 50%, and LoVo extract 750 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP2 concentration in the neat samples was determined to be 1,248 pg/mL in serum, 1,104 pg/mL in plasma (heparin), 1,642 pg/mL in plasma (EDTA), and 1,199 pg/mL in plasma (citrate).
-
Interpolated concentrations of spiked FABP2 in human urine and RPMI media with 10% FBS.The concentrations of FABP2 were measured in duplicate and interpolated from the FABP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
-
Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP2 concentration was determined to be 1,094 pg/mL with a range of 422 – 3,639 pg/mL.
-
Human FABP2 Competitor Std Curve ComparisonStandard curve comparison between human FABP2 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
-
Sandwich ELISA - Human FABP2 ELISA Kit (ab234566)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Sandwich ELISA - Human FABP2 ELISA Kit (ab234566)To learn more about the advantages of recombinant antibodies see here. -
Sandwich ELISA - Human FABP2 ELISA Kit (ab234566)To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab234566 尚未被引用在任何文献中。