人Erythropoietin ELISA试剂盒(EPO) (ab274397)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1 mIU/ml
- Range: 3.125 mIU/ml - 200 mIU/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Erythropoietin ELISA试剂盒(EPO)
参阅全部 EPO 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 8 6.1% 批次间 样品 n Mean SD CV% Serum 3 10% -
样品类型
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
1 mIU/ml -
范围
3.125 mIU/ml - 200 mIU/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 112 109% - 114% Serum 99 98% - 100% Hep Plasma 91 90% - 92% EDTA Plasma 104 96% - 112% Cit plasma 98 86% - 111% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human Erythropoietin ELISA kit (ab274397) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Erythropoietin protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Erythropoietin with 1 mIU/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Erythropoietin is the principal hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Erythropoietin binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3. Erythropoietin is a secreted protein produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. Human erythropoietin is used for the treatment of anemia. Variations affecting the human EPO gene are associated with microvascular complications of diabetes 2. Human Erythropoietin has 83% amino acids sequence identity to rat Erythropoietin, and 80% identity to the mouse Erythropoietin.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10 x 96 tests 1 x 384 tests 10X Human Erythropoietin (EPO) Capture Antibody 1 x 600µl 1 x 6000µl 1 x 600µl 10X Human Erythropoietin (EPO) Detector Antibody 1 x 600µl 1 x 6000µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 200ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 0 x 0 unit 1 unit Antibody Diluent 5BI 1 x 6ml 10 x 6ml 1 x 6ml Human Erythropoietin (EPO) Lyophilized Recombinant Protein 2 vials 2 x 10 vials 2 vials Plate Seal 1 unit 1 x 10 units 1 unit Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 x 10 units 0 x 0 unit Stop Solution 1 x 12ml 1 x 120ml 2 x 12ml TMB Development Solution 1 x 12ml 1 x 120ml 2 x 12ml -
研究领域
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相关性
Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. -
细胞定位
Secreted -
别名
- EP
- EPO
- EPO alpha
see all -
数据库链接
- Entrez Gene: 2056 Human
- Omim: 133170 Human
- SwissProt: P01588 Human
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab274397于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Interpolated concentrations of human Erythropoietin in HepG2 supernatant in 96-well vs. 384-well plates.Interpolated concentration of native Erythropoietin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% HepG2 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Example of human Erythropoietin standard curve in Sample Diluent NS in 96-well vs. 384-well plate.Example of human Erythropoietin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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. Example of human Erythropoietin standard curve in Sample Diluent NS.The Erythropoietin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Erythropoietin in human serum, plasma and HepG2 cell culture supernatant samples.The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, Plasma (EDTA) 100%, plasma (heparin) 100% and Hep G2 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 16 mIU/mL in neat serum, 24 mIU/mL in neat plasma (citrate), 20 mIU/mL in neat plasma (EDTA), 20 mIU/mL in neat plasma (heparin) and 66 mIU/mL in neat Hep G2 supernatant.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 23.4 mIU/mL with a range of 3.3 – 75.7 mIU/mL.
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Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (ab274397)To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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