人ErbB3 ELISA试剂盒(HER3) (ab275101)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 27.6 pg/ml
- Range: 109.4 pg/ml - 7000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
-
产品名称
人ErbB3 ELISA试剂盒(HER3)
参阅全部 ErbB3 / HER3 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 8 3.8% 批次间 样品 n Mean SD CV% Serum 3 4.8% -
样品类型
Cell culture supernatant, Saliva, Urine, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
27.6 pg/ml -
范围
109.4 pg/ml - 7000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 83 82% - 83% Saliva 106 104% - 107% Urine 90 83% - 93% Serum 94 92% - 96% Cell Lysate 97 90% - 106% Hep Plasma 114 112% - 117% EDTA Plasma 91 88% - 94% Cit plasma 92 88% - 94% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human ErbB 3 ELISA kit (ab275101) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human ErbB 3 protein in serum, plasma, cell culture supernatant, urine, saliva and cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ErbB 3 with 27.6 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
说明
Receptor tyrosine-protein kinase ErbB 3, also known human epidermal growth factor receptor 3 (HER3), is encoded by the ERBB3 gene in which is a member of the EGFR family. ErbB 3 is a type I transmembrane glycoprotein that binds to neuregulins NRG1 and NRG2, but lacks a working kinase domain. ErbB 3 is capable of heterodimerization of its fellow ErbB family members, most notably with ErbB 2 for signaling. Moreover, ErbB 3 is vital in the development of Schwann cells, neural crest differentiation, sympathetic nervous system, and heart values. Multiple studies have shown ErbB 3 to play roles in cellular growth, proliferation, differentiation, promotion and metastasis of cancer, and chemotherapeutic resistance.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human ErbB 3 (HER3) Capture Antibody 1 x 600µl 10X Human ErbB 3 (HER3) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human ErbB 3 (HER3) Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
-
功能
Binds and is activated by neuregulins and NTAK. -
组织特异性
Epithelial tissues and brain. -
疾病相关
Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
发展阶段
Overexpressed in a subset of human mammary tumors. -
结构域
The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins. -
翻译后修饰
Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
-
别名
- c erbB 3
- c erbB3
- Erb b2 receptor tyrosine kinase 3
see all -
数据库链接
- Entrez Gene: 2065 Human
- Omim: 190151 Human
- SwissProt: P21860 Human
- Unigene: 118681 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab275101于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
-
SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human ErbB 3 standard curve in Sample Diluent NS.The ErbB 3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native ErbB 3 in human serum, plasma and cell culture supernatant samples.The concentrations of ErbB 3 were measured in duplicates, interpolated from the ErbB 3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, plasma (heparin) 25% and MCF-7 supernatant neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ErbB 3 concentration was determined to be 4,877 pg/mL in serum, 3,874 pg/mL in plasma (citrate), 4,148 pg/mL in plasma (EDTA), 3,783 pg/mL in plasma (heparin), and 301.2 pg/mL in MCF-7 supernatant.
-
Interpolated concentrations of native ErbB 3 in human MCF-7 based on an 800 µg/mL extract load.The concentrations of ErbB 3 were measured in duplicate and interpolated from the ErbB 3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ErbB 3 concentration was determined to be 3,799 pg/mL in MCF-7 cell extract.
-
Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ErbB 3 concentration was determined to be 4561 pg/mL with a range of 3275 pg/mL – 6077 pg/mL.
-
Sandwich ELISA - Human ErbB3 / HER3 ELISA Kit (HER3) (ab275101)To learn more about the advantages of recombinant antibodies see here.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab275101 尚未被引用在任何文献中。