人Entactin ELISA试剂盒(NID1) (ab113338)
Key features and details
- Sensitivity: 0.1 ng/ml
- Range: 0.123 ng/ml - 30 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Entactin ELISA试剂盒(NID1)
参阅全部 Entactin/NID 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 0.1 ng/ml -
范围
0.123 ng/ml - 30 ng/ml -
回收率
> 100 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 125.7 111% - 138% Serum 132.3 123% - 145% Plasma 131.4 122% - 147% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Entactin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Entactin in serum, plasma, cell culture supernatants.
This assay employs an antibody specific for Human Entactin coated on a 96-well plate. Standards and samples are pipetted into the wells and Entactin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Entactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Entactin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Optimization may be required with urine samples.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated anti-Human Entactin 2 vials Entactin Microplate (12 x 8 strips) 1 unit Recombinant Human Entactin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions. -
序列相似性
Contains 6 EGF-like domains.
Contains 4 LDL-receptor class B repeats.
Contains 1 NIDO domain.
Contains 1 nidogen G2 beta-barrel domain.
Contains 1 thyroglobulin type-1 domain. -
翻译后修饰
N- and O-glycosylated. -
细胞定位
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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别名
- Enactin
- Ent
- Entactin
see all -
数据库链接
- Entrez Gene: 4811 Human
- Entrez Gene: 4811 Human
- Omim: 131390 Human
- SwissProt: P14543 Human
- SwissProt: P14543 Human
- Unigene: 356624 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113338于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Representative Standard Curve
Representative Standard Curve using ab113338
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab113338 尚未被引用在任何文献中。