人EFEMP1 ELISA试剂盒(Fibulin-3)
Human EFEMP1 ELISA Kit (Fibulin-3)
- Recombinant
- SimpleStep
- 了解详情
Be the first to review this product! Submit a review
|
(2 Publications)
Human EFEMP1 ELISA Kit (Fibulin-3) is a single-wash 90-min Simplestep used to quantify Human EFEMP1 (Fibulin-3) with a sensitivity of 42 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
查看别名
FBLN3, FBNL, EFEMP1, EGF-containing fibulin-like extracellular matrix protein 1, Extracellular protein S1-5, Fibrillin-like protein, Fibulin-3, FIBL-3
- sELISA
Supplier Data
Sandwich ELISA - Human EFEMP1 ELISA Kit (Fibulin-3) (AB269552)
Interpolated concentrations of native EFEMP1 in human CSF.
The concentrations of EFEMP1 were measured in duplicates, interpolated from the EFEMP1 standard curves and corrected for sample dilution; Undiluted CSF is 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EFEMP1 concentration was determined to be 91 ng/ml.
- sELISA
Supplier Data
Sandwich ELISA - Human EFEMP1 ELISA Kit (Fibulin-3) (AB269552)
Interpolated concentrations of native EFEMP1 in human serum and plasma samples.
The concentrations of EFEMP1 were measured in duplicates, interpolated from the EFEMP1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 400, plasma (citrate) 1 : 800, plasma (heparin) 1 : 400, plasma (EDTA) 1 : 400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EFEMP1 concentration was determined to be 742 ng/mL in serum, 1672 ng/mL in plasma (citrate), 1072 ng/mL in plasma (Heparin), and 997 ng/mL in plasma (EDTA).
- sELISA
Supplier Data
Sandwich ELISA - Human EFEMP1 ELISA Kit (Fibulin-3) (AB269552)
Example of human EFEMP1 standard curve in Sample Diluent NS.
The EFEMP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
反应性数据
产品详情
Human EFEMP1 ELISA Kit (Fibulin-3) (ab269552) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of EFEMP1 (Fibulin-3) protein in serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human EFEMP1 (Fibulin-3) with 42 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
EFEMP1, EGF-containing fibulin-like extracellular matrix protein 1, is a secreted extracellular glycoprotein in the fibulin family. It is a key regulator of cell-to-cell and cell-to-matrix adhesion. It is currently under investigation as a pharmacological target for a host of cancers. The antibodies for this kit were raised against recombinant protein representing the mature full-length human protein.
精确度
回收率
规格
性能和储存信息
运输条件
推荐的短期储存条件
推荐的长期储存条件
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
EFEMP1/Fibulin-3 participates in ECM modulation and cell signaling. It interacts with other ECM components such as fibronectin and collagen contributing to structural assembly and stability. Although not prominently part of multi-protein complexes Fibulin-3 influences cellular adhesion and migration impacting tissue development and repair mechanisms. Researchers observe its expression during embryogenesis suggesting a role in developmental processes.
Pathways
EFEMP1/Fibulin-3 integrates into matrix-related pathways impacting tissue architecture. It plays a role in the EGF (Epidermal Growth Factor) signaling pathway which is important for various cell survival and proliferation processes. Fibulin-3 exhibits functional relationships with proteins like fibronectin and integrins within these pathways highlighting its importance in mediating cell-matrix interactions and influencing signal transduction cascades.
靶点信息
文献 (2)
Recent publications for all applications. Explore the full list and refine your search
iScience 27:108930 PubMed38333700
2024
Applications
Unspecified application
Species
Unspecified reactive species
Journal of experimental & clinical cancer research : CR 42:328 PubMed38031171
2023
Applications
Unspecified application
Species
Unspecified reactive species
搭配使用产品
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com