人DPP4 (CD26) ELISA试剂盒
Human DPP4 (CD26) ELISA Kit
- Recombinant
- SimpleStep
- 了解详情
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Human DPP4 (CD26) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human DPP4 (CD26) with a sensitivity of 12.82 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
查看别名
CD26, ADCP2, DPP4, Dipeptidyl peptidase 4, ADABP, Adenosine deaminase complexing protein 2, Dipeptidyl peptidase IV, T-cell activation antigen CD26, TP103, ADCP-2, DPP IV
- sELISA
Unknown
Sandwich ELISA - Human DPP4 (CD26) ELISA Kit (AB252365)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD26 concentration was determined to be 300.8 ng/mL with a range of 200.7 – 421.5 ng/mL.
- sELISA
Unknown
Sandwich ELISA - Human DPP4 (CD26) ELISA Kit (AB252365)
Example of human CD26 standard curve in Sample Diluent NS.
The CD26 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Unknown
Sandwich ELISA - Human DPP4 (CD26) ELISA Kit (AB252365)
Interpolated concentrations of native CD26 in human male urine, female saliva, PBMC cell culture supernatant stimulated for 46 hours with 1.5% PHA-M, and HepG2 cell culture supernatant samples.
The concentrations of CD26 were measured in duplicates, interpolated from the CD26 standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 12.5%, saliva 50%, PBMC supernatant 100%, and HepG2 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD26 concentration was determined to be 36.1 ng/mL in urine, 13.5 ng/ml in saliva, 1.0 ng/ml in stimulated PBMC supernatant, and 5.8 ng/ml in HepG2 supernatant.
- sELISA
Unknown
Sandwich ELISA - Human DPP4 (CD26) ELISA Kit (AB252365)
Human CD26 Standard Curve Comparison.
Standard Curve comparison between human CD26 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
- sELISA
Unknown
Sandwich ELISA - Human DPP4 (CD26) ELISA Kit (AB252365)
Interpolated concentrations of native CD26 in human serum and plasma samples.
The concentrations of CD26 were measured in duplicates, interpolated from the CD26 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 100, plasma (citrate) 1 : 100, plasma (heparin) 1 : 100, and plasma (EDTA) 1 : 100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD26 concentration was determined to be 570.0 ng/mL in serum, 470.9 ng/mL in plasma (citrate), 514.4 ng/ml in plasma (heparin), and 524.8 ng/ml in plasma (EDTA).
搭配使用产品
反应性数据
产品详情
Human CD26 ELISA Kit (ab252365) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD26 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, saliva, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD26 with 12.82 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CD26, also known as DPP4 (Dipeptidyl Peptidase IV), is a glycoprotein receptor essential for T cell receptor-mediated T cell activation. CD26 is a serine protease that cleaves Xaa-Pro dipeptides from the N terminus of oligo-and polypeptides. Involved in a variety of cellular processes, CD26 is a therapeutic target in clinical trials for type 2 diabetes.
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性能和储存信息
运输条件
推荐的短期储存条件
推荐的长期储存条件
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
DPP4 plays a significant role in glucose metabolism and immune function. The enzyme interacts with members of the immune system and regulates proteins such as chemokines growth factors and neuropeptides. DPP4 acts independently and is not known to be part of a larger complex. It modulates biological processes by inactivating peptide hormones and signaling molecules which impacts glucose homeostasis and immune responses.
Pathways
DPP4 impacts the incretin signaling pathway and the immune response pathway. Incretin hormones like glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are substrates of DPP4 and play important roles in glucose metabolism. DPP4 also interacts with proteins such as adenosine deaminase (ADA) which impacts T-cell function and immune regulation. The regulation of these pathways by DPP4 influences metabolic and immune health.
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