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AB315786

人DNase I ELISA试剂盒

Human DNase I ELISA Kit

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Human DNase I ELISA Kit is a single-wash 90-min SimpleStep ELISA used to quantify Human DNase I with a sensitivity of 2.2 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab327311

查看别名

DNL1, DRNI, DNASE1, Deoxyribonuclease-1, Deoxyribonuclease I, DNase I

3 Images
Sandwich ELISA - Human DNase I ELISA Kit (AB315786)
  • sELISA

Supplier Data

Sandwich ELISA - Human DNase I ELISA Kit (AB315786)

Interpolated concentrations of DNase I in normal human serum donors

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DNase I concentration was determined to be 4,675 pg/mL with a range of 1,271 – 12,993 pg/mL.

Sandwich ELISA - Human DNase I ELISA Kit (AB315786)
  • sELISA

Supplier Data

Sandwich ELISA - Human DNase I ELISA Kit (AB315786)

Interpolated concentrations of human DNase I in serum, plasma (citrate), plasma (EDTA), plasma (heparin), and urine

Interpolated concentration of native DNase I was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, plasma (heparin) 25%, and urine 1%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human DNase I ELISA Kit (AB315786)
  • sELISA

Supplier Data

Sandwich ELISA - Human DNase I ELISA Kit (AB315786)

Example of human DNase I standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Urine, Cell culture media

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 2.2 pg/mL

范围

15.625 - 1000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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搭配使用产品

Assay Kits

AB327311

Human DNase I ELISA Kit, Chemiluminescent

SimpleStep Ignite™ ELISA|Recombinant

Sandwich ELISA - Human DNase I ELISA Kit, Chemiluminescent (AB327311)

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  • 0
Assay Kits

AB234056

DNase I Assay Kit (Fluorometric)

Functional Studies - DNase I Assay Kit (Fluorometric) (AB234056)

  • 4
  • 1
Proteins & Peptides

AB275555

Recombinant human DNase I protein (Active)

Functional Studies - Recombinant human DNase I protein (Active) (AB275555)

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  • 0
Assay Kits

AB196263

Human IgA ELISA Kit

Recombinant|SimpleStep

ELISA - Human IgA ELISA Kit (AB196263)

  • 4
  • 1
Assay Kits

AB189576

Human Apolipoprotein AI ELISA Kit

SimpleStep

ELISA - Human Apolipoprotein AI ELISA Kit (AB189576)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human DNase I SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DNase I protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Urine, Cell culture media. Quantitate Human DNase I with 2.2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]
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回收率

[ { "sample": "Serum", "range": "93 - 99 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "86 - 93 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "92 - 100 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "83 - 109 %", "average": "= 96" }, { "sample": "Urine", "range": "95 - 107 %", "average": "= 101" }, { "sample": "Cell culture media", "range": "78 - 88 %", "average": "= 82" } ]
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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed : 11241278, PubMed : 2251263, PubMed : 2277032). Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity). Among other functions, seems to be involved in cell death by apoptosis (PubMed : 11241278). Binds specifically to G-actin and blocks actin polymerization (By similarity). Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).
See full target information DNASE1
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websiteProtocolBooklet
en
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