人DAP12 ELISA试剂盒(ab317544)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2 pg/ml
- Range: 15.625 pg/ml - 1000 pg/ml
- Sample type: Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人DAP12 ELISA试剂盒
参阅全部 DAP12 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Extract 8 6.1% 批次间 样品 n Mean SD CV% Extract 3 1.9% -
样品类型
Tissue Extracts, Cell Lysate -
检测类型
Sandwich (quantitative) -
灵敏度
2 pg/ml -
范围
15.625 pg/ml - 1000 pg/ml -
回收率
103 %
特定样本回收率 样品类型 平均% 范围 Tissue Extracts 97 92% - 106% Cell Lysate 103 95% - 109% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human DAP12 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DAP12 protein in Cell Lysate, Tissue extracts. Quantitate Human DAP12 with 2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
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说明
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 10 x 96 tests 1 x 96 tests 1 x 384 tests 10X Human DAP12 Capture Antibody 10 x 600µl 1 x 600µl 1 x 600µl 10X Human DAP12 Detector Antibody 10 x 600µl 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 200ml 1 x 20ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 0 x 0 unit 1 unit 5X Cell Extraction Buffer PTR (ab193970) 2 x 50ml 1 x 10ml 1 x 50ml Antibody Diluent CPR2 10 x 6ml 1 x 6ml 1 x 6ml Human DAP12 Lyophilized Recombinant Protein 20 vials 2 vials 2 vials Plate Seal 10 units 1 unit 1 unit Sample Diluent NS (ab193972) 0 x 0ml 1 x 12ml 0 x 0ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 10 units 1 unit 0 x 0 unit Stop Solution 1 x 120ml 1 x 12ml 2 x 12ml TMB Development Solution 1 x 120ml 1 x 12ml 2 x 12ml -
研究领域
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功能
Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. -
组织特异性
Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Expressed in hematological cells and tissues such as peripheral blood leukocytes and spleen. Also found in bone marrow, lymph nodes, placenta, lung and liver. Expressed at lower levels in different parts of the brain especially in the basal ganglia and corpus callosum. -
疾病相关
Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. -
序列相似性
Belongs to the TYROBP family. -
翻译后修饰
Tyrosine phosphorylated. -
细胞定位
Membrane. - Information by UniProt
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别名
- DAP 12
- DAP12
- DNAX activation protein 12
see all -
数据库链接
- Entrez Gene: 7305 Human
- Omim: 604142 Human
- SwissProt: O43914 Human
- Unigene: 515369 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317544于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Example of human DAP12 standard curve in 1X Cell Extraction Buffer PTR.
Example of human DAP12 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of human DAP12 in THP-1 cell extract.Interpolated concentration of native DAP12 was measured in duplicate at different sample concentrations. Undiluted samples are 60 µg/mL THP-1 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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Interpolated concentrations of human DAP12 in THP-1 cell extract in 96-well vs. 384-well plates.Interpolated concentration of native DAP12 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 60 µg/mL THP-1 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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Example of human DAP12 standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate.Example of human DAP12 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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Human DAP12 SimpleStep ELISA Kit -
Human DAP12 SimpleStep ELISA Kit -
Human DAP12 SimpleStep ELISA Kit
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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