人Cytokeratin 18 Fragment ELISA试剂盒(CK18-30)
Human Cytokeratin 18 Fragment ELISA Kit (CK18-30)
- Recombinant
- SimpleStep
- 了解详情
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(3 Publications)
Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) is a single-wash 90-min Simplestep used to quantify Human Cytokeratin 18 Fragment (CK18-30) with a sensitivity of 22.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
查看别名
CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18
- ELISA
Supplier Data
ELISA - Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) (AB254515)
- sELISA
Supplier Data
Sandwich ELISA - Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) (AB254515)
Interpolated concentrations of native Cytokeratin 18 Fragment (CK18-30) in human HeLa cell extract and extract based on a 12.5 μg/mL and 1.56 µg/mL extract load (respectively).
The concentrations of Cytokeratin 18 Fragment were measured in duplicate and interpolated from the Cytokeratin 18 Fragment standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 18 Fragment concentration was determined to be 42.1 pg/mL / μg/mL in untreated and 394 pg/mL / μg/mL in STS treated HeLa cell extract.
- sELISA
Supplier Data
Sandwich ELISA - Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) (AB254515)
Example of human Cytokeratin 18 Fragment (CK18-30) standard curve in Sample Diluent NS.
The Cytokeratin 18 Fragment standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) (AB254515)
Interpolated concentrations of spike Cytokeratin 18 Fragment (CK18-30) in human serum and plasma samples.
The concentrations of Cytokeratin 18 Fragment were measured in duplicates, interpolated from the Cytokeratin 18 Fragment standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma – citrate 25%, plasma- EDTA 25%, and plasma – heparin 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) (AB254515)
Interpolated concentrations of native Cytokeratin 18 Fragment (CK18-30) in human HeLa cell culture supernatant samples.
The concentrations of Cytokeratin 18 Fragment were measured in duplicates, interpolated from the Cytokeratin 18 Fragment standard curves and corrected for sample dilution. Undiluted samples are as follows : untreated 100% and staurosporine treated (4hr at 1 μM) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 18 Fragment concentration was determined to be 239 pg/mL in untreated and 400 pg/mL in STS treated cell culture supernatant.
反应性数据
产品详情
Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) ab254515 is a rapid single-wash 90-min Sandwich ELISA to measure Human Cytokeratin 18 Fragment (CK18-30)in cell culture supernatant, cell lysate, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 22.8 pg/mL.
How the assay works
Human Cytokeratin 18 Fragment (CK18-30)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) ab254515 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Cytokeratin 18 Fragment is a degradation product of Cytokeratin 18. Cytokeratin 18 is involved in the uptake of thrombin-antithrombin complexes by hepatic cells. Cytokeratin 18 becomes cleaved during apoptosis resulting in a 18kDa fragment, which can be used as a measure of cell death and a biomarker for liver disease. ab254515 recognizes this fragment, and specifically recognizes the cleavage site (M30) neo-epitope and does not recognize the full-length protein.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.
Pathways
Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.
靶点信息
文献 (3)
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