AB227896

人Cytokeratin 18 ELISA试剂盒

Human Cytokeratin 18 ELISA Kit

Recombinant
SimpleStep
了解详情

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(3 Publications)

Human Cytokeratin 18 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Cytokeratin 18 with a sensitivity of 3.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

8 Images

ELISA - Human Cytokeratin 18 ELISA Kit (AB227896)

ELISA

Supplier Data

ELISA - Human Cytokeratin 18 ELISA Kit (AB227896)

sELISA

Supplier Data

Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (AB227896)

Interpolated concentrations of native Cytokeratin 18 in Hela cell extract and Human Liver tissue extract.

The concentrations of Cytokeratin 18 were measured in duplicate and interpolated from the Cytokeratin 18 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 18 concentration was determined to be 0.89 pg / μg Hela extract and 35 pg/ μg Liver extract.

sELISA

Supplier Data

Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (AB227896)

Interpolated concentrations of native Cytokeratin 18 in Human cell culture supernatant sample.

The concentrations of Cytokeratin 18 were measured in duplicates, interpolated from the Cytokeratin 18 standard curves and corrected for sample dilution. Undiluted samples are as follows : Hela cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 18 concentration was determined to be 1000 pg/mL in Hela cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (AB227896)

Example of Human Cytokeratin 18 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (AB227896)

Serum from ten individual healthy Human female donors were measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). 5/10 donors were below the detectable dose. The mean of the detectable donors Cytokeratin 18 concentration was determined to be 59 pg/mL with a range of 31– 79 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (AB227896)

Interpolated concentrations of spiked Cytokeratin 18 in Human serum and plasma samples.

sELISA

Supplier Data

Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (AB227896)

Interpolated concentrations of native Cytokeratin 18 in Human serum and plasma samples.

The concentrations of Cytokeratin 18 were measured in duplicates, interpolated from the Cytokeratin 18 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (EDTA) 50%, plasma (heparin) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 18 concentration was determined to be 23 pg/mL in serum, 29pg/mL in plasma (EDTA), 96 pg/mL in plasma (heparin), and 33 pg/mL in plasma (citrate).

sELISA

Supplier Data

Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (AB227896)

Example of Human Cytokeratin 18 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 3.4 pg/mL

范围

15.6 - 1000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human Cytokeratin 18 ELISA Kit ab227896 is a rapid single-wash 90-min Sandwich ELISA to measure Human Cytokeratin 18 in cell culture extracts, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 3.4 pg/mL.

How the assay works

Human Cytokeratin 18 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Cytokeratin 18 ELISA Kit ab227896 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.4" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" } ]

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回收率

[ { "sample": "Serum", "range": "99 - 102 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "97 - 100 %", "average": "= 99" }, { "sample": "Cell culture extracts", "range": "106 - 115 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "97 - 99 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "93 - 97 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "107 - 110 %", "average": "= 109" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab227896-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB227896-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB227896-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB227896-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB227896-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB227896-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent LS", "number":"AB227896-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cytokeratin 18 Capture Antibody", "number":"AB227896-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB227896-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Cytokeratin 18 Lyophilized Recombinant Protein", "number":"AB227896-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB227896-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cytokeratin 18 Detector Antibody", "number":"AB227896-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB227896-CMP07", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

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产品实验方案

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靶点信息

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

See full target information KRT18

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Toxicology reports 14:102009 PubMed40226810

2025

Potential amelioration of liver function by low-dose tolvaptan in heart failure patients.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuaki Mino,Kohei Hoshikawa,Takafumi Naito,Shunta Akutsu,Yumi Imoto,Emi Nakatsugawa,Masao Saotome,Yuichiro Maekawa,Junichi Kawakami

Cell death and differentiation 29:1240-1254 PubMed34997207

2022

SARS-CoV-2 non-structural protein 6 triggers NLRP3-dependent pyroptosis by targeting ATP6AP1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Sun,Yingzhi Liu,Ziheng Huang,Wenye Xu,Wei Hu,Lina Yi,Zhe Liu,Hung Chan,Judeng Zeng,Xiaodong Liu,Huarong Chen,Jun Yu,Francis Ka Leung Chan,Siew Chien Ng,Sunny Hei Wong,Maggie Haitian Wang,Tony Gin,Gavin Matthew Joynt,David Shu Cheong Hui,Xuan Zou,Yuelong Shu,Christopher Hon Ki Cheng,Shisong Fang,Huanle Luo,Jing Lu,Matthew Tak Vai Chan,Lin Zhang,William Ka Kei Wu

Oncology letters 22:705 PubMed34457060

2021

Role of JNK activation in paclitaxel-induced apoptosis in human head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Yan Lan,Ying-Hui Chen,Cheng Liu,Kuo-Lung Tung,Yen-Ting Wu,Sheng-Chieh Lin,Chin-Han Wu,Hong-Yi Chang,Yung-Chia Chen,Bu-Miin Huang

View all publications

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websiteProtocolBooklet

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

人Cytokeratin 18 ELISA试剂盒

Human Cytokeratin 18 ELISA Kit

ELISA - Human Cytokeratin 18 ELISA Kit (AB227896)

Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (AB227896)

Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (AB227896)

Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (AB227896)

Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (AB227896)

Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (AB227896)

Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (AB227896)

Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (AB227896)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台