人Cyt C Reductase ELISA试剂盒(Complex III) Profiling ELISA试剂盒(ab124537)
Key features and details
- Sensitivity: 4 µg/ml
- Range: 6 µg/ml - 200 µg/ml
- Sample type: Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (qualitative)
- Reacts with: Human
概述
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产品名称
人Cyt C Reductase ELISA试剂盒(Complex III) Profiling ELISA试剂盒
参阅全部 Complex III 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 8 4.7% 批次间 样品 n Mean SD CV% Overall 6 19.7% -
样品类型
Tissue Extracts, Cell Lysate -
检测类型
Sandwich (qualitative) -
灵敏度
4 µg/ml -
范围
6 µg/ml - 200 µg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
产品概述
Ubiquinol-cytochrome-c reductase (Complex III) is the third enzyme of the oxidative phosphorylation (OXPHOS) system within the mitochondrial inner membrane. Ubiquinol-cytochrome-c reductase is a large protein complex of approximately 250,000 MW made up of 11 different subunits. The enzyme forms a dimer in the mitochondrial inner membrane. The catalytic subunit cytochrome b is encoded on mitochondrial DNA (mtDNA). All other subunits are encoded by nuclear genomic DNA, made in the cytosol, and translocated into the organelle for assembly at the inner membrane. Complex III catalyses the pumping of protons across the inner membrane and transfer of electrons from ubiquinol to cytochrome c by a mechanism known as the Q cycle in which ubiquinol (Q) the electron carrier exists as a reduced, partially reduced or oxidized form.
ab124537 Cyt C Reductase (Complex III) human profiling kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the comparison of Complex III levels or profile in cell and tissue lysates. The assay employs a human Complex III specific antibody coated onto microplate well plate strips.
Samples are pipetted into the wells and Complex III present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-Complex III detector antibody is added. After washing away unbound detector antibody, an HRP-conjugated secondary antibody specific for the detector antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of Complex III bound. The developing blue color is measured at 600 nm.
Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Species– Human. Rat and Mouse samples are not appropriate, other species are untested.
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说明
Store all components at 4°C. This kit is stable for 6 months from receipt. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Blocking Buffer 1 x 6ml 10X Complex III Detector Antibody 1 x 1ml 10X HRP Label 1 x 1ml 20X Buffer 1 x 20ml Complex III Microplate 1 unit Extraction Buffer (ab260490) 1 x 15ml HRP Development Solution 1 x 12ml -
研究领域
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相关性
Complex II (succinate-ubiquinone oxidoreductase), one of the mitochondrial respiratory chain complexes, transfers electrons from succinate generated during the citric acid cycle to Complex III (ubiquinolcytochrome c oxidoreductase), via a mobile electron shuttle, ubiquinone. Complex III transfers electrons to Complex IV (cytochrome c oxidase) via another mobile electron shuttle, cytochrome c. -
别名
- coenzyme Q : cytochrome c — oxidoreductase
- cytochrome bc1 complex
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数据库链接
- Entrez Gene: 7384 Human
- Entrez Gene: 7385 Human
- Entrez Gene: 1537 Human
- Entrez Gene: 7386 Human
- Entrez Gene: 4519 Human
- Entrez Gene: 7381 Human
- Entrez Gene: 7388 Human
- Entrez Gene: 27089 Human
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124537于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Sandwich ELISA - Human Cyt C Reductase (Complex III) Profiling ELISA Kit (ab124537)
Example control sample curve of serially titrated HepG2 extract in the working range of the assay.
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Sandwich ELISA - Human Cyt C Reductase (Complex III) Profiling ELISA Kit (ab124537)Human HepG2 cells were cultured in chloramphenicol for 6 days to ensure a significant effect on mitochondrial DNA replication and mitochondrial protein translation, respectively. The antibiotic inhibited mitochondrial protein translation and assembly of Complexes I and IV but had no significant effect on Complex II, III or V. -
Sandwich ELISA - Human Cyt C Reductase (Complex III) Profiling ELISA Kit (ab124537)Human HepG2 cells were cultured in NARTI Zalcitabine (ddC) for 6 days to ensure a significant effect on mitochondrial DNA replication and mitochondrial protein translation, respectively. The drug reduced mitochondrial DNA levels and hence mitochondrial protein expression. As a consequence the assembly of Complexes I, III and IV were severely affected. Note that loss of the two small mitochondrial DNA encoded subunits of Complex V (ATP synthase) does not affect overall assembly. Interestingly an increase in Complex II was induced as a consequence of I, III, IV loss possibly to up regulate mitochondrial citric acid cycle function.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab124537 被引用在 6 文献中.
- Huang GD et al. Moschamindole induces glioma cell apoptosis by blocking Mia40-dependent mitochondrial intermembrane space assembly and oxidative respiration. Phytother Res 35:3390-3405 (2021). PubMed: 33856743
- Cui P et al. STAT3 inhibition induced temozolomide-resistant glioblastoma apoptosis via triggering mitochondrial STAT3 translocation and respiratory chain dysfunction. Cell Signal 71:109598 (2020). PubMed: 32165236
- Jaworski S et al. Degradation of Mitochondria and Oxidative Stress as the Main Mechanism of Toxicity of Pristine Graphene on U87 Glioblastoma Cells and Tumors and HS-5 Cells. Int J Mol Sci 20:N/A (2019). PubMed: 30717385
- Khasawneh RR et al. Cross talk between 26S proteasome and mitochondria in human mesenchymal stem cells' ability to survive under hypoxia stress. J Physiol Sci 69:1005-1017 (2019). PubMed: 31679117
- Ye Q et al. Mitochondrial Effects of PGC-1alpha Silencing in MPP(+) Treated Human SH-SY5Y Neuroblastoma Cells. Front Mol Neurosci 10:164 (2017). ELISA ; Rat . PubMed: 28611589
- Cherry AB et al. Induced Pluripotent Stem Cells with a Pathological Mitochondrial DNA Deletion. Stem Cells N/A:N/A (2013). ELISA ; Human . PubMed: 23400930