JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314725

人Cystatin SN/CST1 ELISA试剂盒

Human Cystatin SN/CST1 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Cystatin SN/CST1 ELISA Kit is a Sandwich ELISA for the measurement of Human Cystatin SN/CST1 in Human in Biofluids, Cell Culture Media samples.

查看别名

Cystatin-SN, Cystain-SA-I, Cystatin-1, Salivary cystatin-SA-1, CST1

1 Images
Sandwich ELISA - Human Cystatin SN/CST1 ELISA Kit (AB314725)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cystatin SN/CST1 ELISA Kit (AB314725)

Example of standard curve using ab314725. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

关键信息

检测方法

Colorimetric

样品类型

Serum, Plasma, Cell culture supernatant

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 0.2 ng/mL

范围

0.2 - 50 ng/mL

检测平台

Pre-coated microplate (12 x 8 well strips)

style
left-column

搭配使用产品

Assay Kits

AB277447

Human Galectin 10 ELISA Kit

ELISA - Human Galectin 10 ELISA Kit (AB277447)

  • 0
  • 0
Assay Kits

AB283970

Human Cystatin S ELISA Kit

Sandwich ELISA - Human Cystatin S ELISA Kit (AB283970)

  • 0
  • 0
Biochemicals

AB120056

Staurosporine, Protein kinase inhibitor

Chemical Structure - Staurosporine, Protein kinase inhibitor (AB120056)

  • 0
  • 0
Assay Kits

AB314724

Human Cystatin SA/CST2 2 ELISA Kit

Sandwich ELISA - Human Cystatin SA/CST2 2 ELISA Kit (AB314724)

  • 0
  • 0
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

Human Cystatin SN/CST1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Cystatin SN/CST1 in serum (human Cystatin SN/CST1 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human Cystatin SN/CST1 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human AMIGO, Aminopeptidase LRAP, Amnionless, Arylsulfatase A, Bcl-w, CD109, CD157, CD34, CD83, CLEC- 1, CMG-2, CREG, Cytokeratin-8, Dectin-1, Desmocollin-3, Endoglycan, Galectin-4, HAPLN1, Jagged 1, Langerin, Lumican, Matriptase, MEP1B, Nectin-3, OX40, OX40 Ligand, p27, Pappalysin-2, Plexin B3, Plexin D1, proGRP, PSA-total, Reg1B, RGM-A, ROBO2, Spinesin, TWEAK R, ULBP-3.

style
left-column

回收率

[ { "sample": "Serum", "range": "105 - 121 %", "average": "= 112.4" }, { "sample": "Plasma", "range": "84 - 131 %", "average": "= 106.3" }, { "sample": "Cell culture media", "range": "109 - 126 %", "average": "= 120.1" } ]
style
left-column

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314725-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314725-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Cystatin SN/CST1 Antibody-coated ELISA Plate", "number":"AB314725-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314725-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314725-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Cystatin SN/CST1 Detection Antibody", "number":"AB314725-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin", "number":"AB314725-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314725-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Cystatin SN/CST1 Protein Standard", "number":"AB314725-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-Well Plate Sealing Film", "number":"AB314725-CMP02", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Cystatin SN also known as CST1 is a cysteine protease inhibitor that belongs to the family of cystatins. This protein has a mass of approximately 16 kDa and is expressed primarily in human saliva as well as other tissues like the nasal mucosa and skin. Its main role involves binding to and inhibiting cathepsins and other cysteine protease enzymes preventing the breakdown of cellular and extracellular proteins. Such inhibition protects tissues from damage and helps maintain normal protein turnover.
Biological function summary

CST1 inhibits proteolytic enzymes that are involved in degrading proteins within cells and tissues. This protein is not part of a larger complex but instead exerts its function through one-on-one interactions with target proteases. Through its inhibitory actions CST1 plays a protective role in various bodily fluids and epithelial surfaces contributing to the body's barrier and immune defense mechanisms against pathogens and tissue damage.

Pathways

CST1 is engaged in the regulation of protease activity which impacts the broader proteolytic pathways that are important to cellular homeostasis. This regulation is linked to the lysosomal cathepsin family and pathways such as the protein metabolism pathway. Related proteins within these pathways include cathepsin L and cathepsin B which are also cysteine proteases that rely on precise regulation by cystatin family proteins like CST1 to prevent excessive protein degradation.

CST1 has associations with conditions like chronic rhinosinusitis and certain cancers. Chronic rhinosinusitis involves excessive inflammation and tissue damage a process where CST1's inhibition of proteases like cathepsin B is important. In cancer abnormal CST1 expression levels have been noted in gastric and colorectal cancers suggesting a connection with tumor growth and metastasis. In these contexts altered expression or function of CST1 may interact with other proteins such as cathepsin L influencing disease progression.
style
left-column

产品实验方案

style
left-column

靶点信息

Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well.
See full target information CST1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com