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AB283970

人Cystatin S ELISA试剂盒

Human Cystatin S ELISA Kit

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Human Cystatin S ELISA Kit is a Sandwich ELISA for the measurement of Human Cystatin S in Human in Cell Culture Media, Biofluids samples.

查看别名

Cystatin-S, Cystatin-4, Cystatin-SA-III, Salivary acidic protein 1, CST4

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Sandwich ELISA - Human Cystatin S ELISA Kit (AB283970)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cystatin S ELISA Kit (AB283970)

Example of Human Cystatin S standard curve. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Cell culture supernatant, Saliva

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 7.2 pg/mL

范围

15.625 - 1000 pg/mL

检测时间

4h

检测平台

Pre-coated microplate (12 x 8 well strips)

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搭配使用产品

Assay Kits

AB314724

Human Cystatin SA/CST2 2 ELISA Kit

Sandwich ELISA - Human Cystatin SA/CST2 2 ELISA Kit (AB314724)

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Assay Kits

AB314725

Human Cystatin SN/CST1 ELISA Kit

Sandwich ELISA - Human Cystatin SN/CST1 ELISA Kit (AB314725)

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Assay Kits

AB201280

Mouse Cystatin C ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Cystatin C ELISA Kit (AB201280)

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Proteins & Peptides

AB151348

Recombinant Human Cystatin S protein

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  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

The Human Cystatin S ELISA Kit (ab283970) is designed for detection of Cystatin-S (CST4) in human saliva and cell culture supernatant samples.

This assay employs a quantitative sandwich enzyme immunoassay technique that measures human CST4 in approximately 4 hours. A polyclonal antibody specific for human CST4 has been pre-coated onto a 96- well microplate with removable strips. CST4 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human CST4, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283970-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Human Cystatin S Microplate", "number":"AB283970-CMP05", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB283970-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent M Concentrate", "number":"AB283970-CMP01", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB283970-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB283970-CMP09", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB283970-CMP06", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB283970-CMP02", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB283970-CMP10", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Cystatin S Antibody", "number":"AB283970-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Cystatin S Standard", "number":"AB283970-CMP03", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
Multi
储存信息
Please refer to protocols
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Cystatin S also known as CST4 is a cysteine protease inhibitor with a molecular mass of approximately 14 kDa. This protein belongs to the cystatin superfamily which is a group of proteins that inhibit proteases such as cathepsins. Cystatin S is mainly expressed in human saliva tears and other bodily fluids where it plays a significant role in protecting tissues from protease activity. Expression levels of cystatin S vary across tissues and can influence its inhibitory functions.
Biological function summary

Cystatin S regulates proteolytic activity by inhibiting enzymes like papain and cathepsins. This protein aids in maintaining the equilibrium between proteases and their inhibitors which is essential for protecting extracellular matrices. Cystatin S does not form part of a larger complex but functions independently to provide defense against unwanted proteolysis in the oral cavity and other environments.

Pathways

Cystatin S interacts with proteolytic pathways that handle extracellular matrix degradation and inflammatory responses. Inhibiting cathepsins within these pathways counters tissue destruction and contributes to cellular homeostasis. Cystatin-related proteins such as cystatin C share similar inhibitory roles indicating their importance in overlapping regulatory networks.

Aberrant expression or function of cystatin S associates with conditions like periodontitis and Sjögren's syndrome. In periodontitis imbalance in protease inhibition leads to periodontal tissue breakdown. Sjögren's syndrome characterized by reduced secretion of tears and saliva links to altered cystatin S levels and its interaction with other protease inhibitors like cystatin C. Both disorders highlight the significance of cystatin S in maintaining tissue integrity.
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产品实验方案

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靶点信息

This protein strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively.
See full target information CST4
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websiteProtocolBooklet
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