AB231929

人Cyclin E1 ELISA试剂盒

Human Cyclin E1 ELISA Kit

Recombinant
SimpleStep
了解详情

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Human Cyclin E1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Cyclin E1 with a sensitivity of 1.2 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

CCNE, CCNE1, G1/S-specific cyclin-E1

4 Images

Sandwich ELISA - Human Cyclin E1 ELISA Kit (AB231929)

sELISA

Supplier Data

Sandwich ELISA - Human Cyclin E1 ELISA Kit (AB231929)

Representative image of Human Cyclin E1 ELISA Kit (ab231929)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human Cyclin E1 ELISA Kit Capture Antibody

- 10X Human Cyclin E1 Detector Antibody

- 10X Human Cyclin E1 ELISA Kit Lyophilised Purified Protein (2 vials)

- Human Cyclin E1 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Unknown

Sandwich ELISA - Human Cyclin E1 ELISA Kit (AB231929)

Interpolated concentrations of native Cyclin E1 in HeLa cell extract based on a 1,500 μg/mL extract load.

The concentrations of Cyclin E1 were measured in duplicate and interpolated from the Cyclin E1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cyclin E1 concentration was determined to be 54 ng/mL in HeLa cell extract.

sELISA

Unknown

Sandwich ELISA - Human Cyclin E1 ELISA Kit (AB231929)

Interpolated concentrations of native Cyclin E1 in JAR cell extract based on a 1,000 μg/mL extract load.

sELISA

Supplier Data

Sandwich ELISA - Human Cyclin E1 ELISA Kit (AB231929)

Example of human Cyclin E1 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Cell culture extracts, Tissue Extracts

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 1.2 ng/mL

范围

3.91 - 250 ng/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human Cyclin E1 ELISA Kit (ab231929) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cyclin E1 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cyclin E1 with 1.2 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

The standard is affinity purified.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12.4" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" } ]

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回收率

[ { "sample": "Cell culture extracts", "range": "98 - 108 %", "average": "= 104" }, { "sample": "Tissue Extracts", "range": "98 - 102 %", "average": "= 101" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab231929-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB231929-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB231929-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB231929-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB231929-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB231929-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB231929-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cyclin E1 Capture Antibody", "number":"AB231929-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB231929-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Cyclin E1 Lyophilized Recombinant Protein", "number":"AB231929-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cyclin E1 Detector Antibody", "number":"AB231929-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB231929-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB231929-CMP02", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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产品实验方案

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靶点信息

Essential for the control of the cell cycle at the G1/S (start) transition.

See full target information CCNE1

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websiteProtocolBooklet

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

人Cyclin E1 ELISA试剂盒

Human Cyclin E1 ELISA Kit

Sandwich ELISA - Human Cyclin E1 ELISA Kit (AB231929)

Sandwich ELISA - Human Cyclin E1 ELISA Kit (AB231929)

Sandwich ELISA - Human Cyclin E1 ELISA Kit (AB231929)

Sandwich ELISA - Human Cyclin E1 ELISA Kit (AB231929)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台