人Cyclin D1 ELISA试剂盒

产品详情

Human Cyclin D1 ELISA Kit (ab214571) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cyclin D1 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cyclin D1 with 33 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Activity of cyclin dependent kinases CDK4 and CDK6 is regulated by the abundance of their cyclin D partners, by phosphorylation, and by association with CDK inhibitors including Kip proteins. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. The inactive ternary complex of cyclin D1/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the dissociation and degradation of p27 concomitant with a rise in cyclin D1 levels to allow G1/S progression. Active cyclin D1-CDK4 complex phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1. The phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Upon withdrawal of growth factors, levels of cyclin D1 protein are reduced via downregulation of protein expression and phosphorylation-dependent degradation.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.