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AB289695

人CXCL11 ELISA试剂盒

Human CXCL11 ELISA Kit

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(2 Publications)

Human CXCL11 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CXCL11 with a sensitivity of 2.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

ITAC, SCYB11, SCYB9B, CXCL11, C-X-C motif chemokine 11, Beta-R1, H174, Interferon gamma-inducible protein 9, Interferon-inducible T-cell alpha chemoattractant, Small-inducible cytokine B11, IP-9, I-TAC

4 Images
Sandwich ELISA - Human CXCL11 ELISA Kit (AB289695)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL11 ELISA Kit (AB289695)

Interpolated concentrations of human CXCL11 in cell culture supernatants.

Interpolated concentration of native CXCL11 was measured in duplicate at different sample concentrations. Undiluted samples are 100% THP-1 supernatant treated with IFN-gamma 200 ng/ml and LPS 50 ng/ml for 24 hours, and 100% THP-1 supernatant untreated. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human CXCL11 ELISA Kit (AB289695)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL11 ELISA Kit (AB289695)

Human CXCL11 standard curve comparison.

Standard curve comparison between the original Human CXCL11 SimpleStep ELISA (ab187392) and current Human CXCL11 SimpleStep ELISA (ab289695).

Sandwich ELISA - Human CXCL11 ELISA Kit (AB289695)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL11 ELISA Kit (AB289695)

Human CXCL11 competitor standard curve comparison.

Standard curve comparison between Human CXCL11 SimpleStep ELISA kit (ab289695) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity

Sandwich ELISA - Human CXCL11 ELISA Kit (AB289695)
  • sELISA

Supplier Data

Sandwich ELISA - Human CXCL11 ELISA Kit (AB289695)

Example of human CXCL11 standard curve in Sample Diluent NS.

Example of human CXCL11 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 2.9 pg/mL

范围

31.25 - 2000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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搭配使用产品

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human CXCL11 ELISA Kit (ab289695) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CXCL11 in serum, plasma (EDTA), plasma (citrate), cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CXCL11 with 2.9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]
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回收率

[ { "sample": "Cell culture supernatant", "range": "85 - 105 %", "average": "= 98" }, { "sample": "Serum", "range": "83 - 89 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "97 - 101 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "99 - 116 %", "average": "= 107" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab289695-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB289695-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB289695-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB289695-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB289695-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB289695-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB289695-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB289695-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CXCL11 Lyophilized Recombinant Protein", "number":"AB289695-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CXCL11 Capture Antibody", "number":"AB289695-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CXCL11 Detector Antibody", "number":"AB289695-CMP02", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CXCL11 also known as I-TAC or Interferon-inducible T-cell alpha chemoattractant is a small cytokine belonging to the CXC chemokine family. It weighs around 8 to 11 kilodaltons. CXCL11 predominantly expresses itself in tissues such as the thymus spleen and peripheral blood leukocytes after stimulation with interferons. Its primary role is attracting activated T-cells especially those expressing CXCR3 receptor contributing to immune surveillance and response.
Biological function summary

The chemokine plays an important role in immune system regulation and inflammation response. It attracts and activates leukocytes including T-cells and natural killer cells driving them to sites of inflammation and injury. CXCL11 is not part of a complex but interacts with other chemokines and cytokines to modulate immune responses. Its expression is upregulated in response to IFN-γ working synergistically with other chemokines like CXCL9 and CXCL10 in inflammatory environments.

Pathways

CXCL11 is integral to the chemotaxis signaling pathway. It influences the migration and activation of immune cells by interacting with the CXCR3 receptor. This pathway shares involvement with proteins such as CXCL10 which also binds to CXCR3 enabling precise directionality and regulation of immune cell movement towards inflammation sites. Additionally CXCL11's participation in the inflammatory response pathway connects it to multiple cellular responses necessary for combating infections.

CXCL11 has been linked to autoimmune diseases and cancer. Its elevated expression is associated with conditions like multiple sclerosis where it may contribute to the pathological migration of immune cells into the central nervous system. In cancer CXCL11 can affect tumor microenvironments by recruiting immune cells with cytotoxic potential although this may also facilitate tumor progression in certain contexts. Proteins related to its disease association include CXCR3 and CXCL10 which together modulate various immunological outcomes in these diseases.
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产品实验方案

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靶点信息

Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
See full target information CXCL11
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer research 84:3728-3742 PubMed39137404

2024

YAP1 Inhibition Induces Phenotype Switching of Cancer-Associated Fibroblasts to Tumor Suppressive in Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hongtao Song,Tong Lu,Donghui Han,Jiayu Zhang,Lunbiao Gan,Chao Xu,Shaojie Liu,Peng Li,Keying Zhang,Zhihao Hu,Hongji Li,Yu Li,Xiaolong Zhao,Jingliang Zhang,Nianzeng Xing,Changhong Shi,Weihong Wen,Fa Yang,Weijun Qin

American journal of translational research 14:6874-6888 PubMed36398225

2022

IFN-γ promotes the development of systemic lupus erythematosus through the IFNGR1/2-PSTAT1-TBX21 signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Chen,Bailing Tian
View all publications
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