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AB264616

人CTLA-4 ELISA试剂盒

Human CTLA-4 ELISA Kit

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Human CTLA-4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CTLA-4 with a sensitivity of 13.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

CD152, CTLA4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4

3 Images
Sandwich ELISA - Human CTLA-4 ELISA Kit (AB264616)
  • sELISA

Supplier Data

Sandwich ELISA - Human CTLA-4 ELISA Kit (AB264616)

Interpolated concentration of native CTLA-4 in human thymus tissue extract based on a 250 μg/mL extract load.

The concentration of CTLA-4 were measured in duplicate and interpolated from the CTLA-4 standard curve. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CTLA-4 concentration was determined to be 66.2 pg/mL in thymus sample

Sandwich ELISA - Human CTLA-4 ELISA Kit (AB264616)
  • sELISA

Supplier Data

Sandwich ELISA - Human CTLA-4 ELISA Kit (AB264616)

Representative image of Human CTLA-4 ELISA Kit (ab264616)

Components shown from left to right :

- Sample diluent NS

- 10X Wash Buffer PT (ab206977)

- 10X Human CTLA-4 Detector Antibody

- Human CTLA-4 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Human CTLA-4 Capture Antibody (Lyophilised) (2 vials)

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human CTLA-4 ELISA Kit (AB264616)
  • sELISA

Supplier Data

Sandwich ELISA - Human CTLA-4 ELISA Kit (AB264616)

Example of human CTLA-4 standard curve in Sample Diluent NS.

The CTLA-4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 13.2 pg/mL

范围

46.88 - 3000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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搭配使用产品

Assay Kits

AB252360

Human PD-1 ELISA Kit

Recombinant|SimpleStep

ELISA - Human PD-1 ELISA Kit (AB252360)

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Assay Kits

AB277712

Human PD-L1 ELISA Kit [28-8]

Recombinant|SimpleStep

ELISA - Human PD-L1 ELISA Kit [28-8] (AB277712)

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Assay Kits

AB282870

Mouse IL-21 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse IL-21 ELISA Kit (AB282870)

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Primary Antibodies

AB270127

Alexa Fluor® 647 Anti-CTLA4 antibody [CAL49]

BOND RX™ Validated|RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CTLA4 antibody [CAL49] (AB270127)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human CTLA-4 ELISA Kit (ab264616) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CTLA-4 protein in cell culture supernatant, cell lysate, cit plasma, edta plasma, hep plasma, serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CTLA-4 with 13.2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Cytotoxic T lymphocyte antigen-4 (CTLA-4) is a transmembrane protein expressed on T regulator cells. CTLA-4 is a negative regulator of T cell activation. CTLA-4 binds markers on antigen presenting cells (APC) including B7, which functions as an immune check point to down regulate the immune response. Blocking CTLA-4 allows for activation and proliferation of more T cells which reduces the mediated immunosuppression. CTLA-4 soluble extracellular domain in serum and plasma is an indication of autoimmune diseases.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" } ]
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回收率

[ { "sample": "Cell culture supernatant", "range": "95 - 98 %", "average": "= 97" }, { "sample": "Serum", "range": "93 - 98 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "87 - 92 %", "average": "= 89" }, { "sample": "Heparin Plasma", "range": "91 - 95 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "88 - 96 %", "average": "= 91" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab264616-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB264616-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB264616-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB264616-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264616-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264616-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB264616-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB264616-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264616-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB264616-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized Human CTLA-4 Capture Antibody", "number":"AB264616-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CTLA-4 Lyophilized Recombinant Protein", "number":"AB264616-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CTLA-4 Detector Antibody", "number":"AB264616-CMP01", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CTLA-4 also known as CD152 is an immune checkpoint receptor with a molecular weight of approximately 34 kDa. This protein is expressed on the surface of T-cells especially after activation and sometimes on regulatory T-cells (Tregs). CTLA-4 competes with CD28 for binding to the same ligands CD80 and CD86 on antigen-presenting cells. Unlike CD28 which stimulates T-cell activation CTLA-4 sends inhibitory signals to downregulate immune responses. By doing this CTLA-4 helps maintain immune system homeostasis and prevents autoimmune reactions.
Biological function summary

The CTLA-4 protein functions as a critical regulator of the immune system. It interacts with CD80/CD86 in a complex that effectively transmits inhibitory signals to T-cells. CTLA-4 controls the amplitude of the initial activation of T-cells ensuring that the body's immune responses are kept under control. In regulatory T-cells CTLA-4 engagement contributes to their immunosuppressive functions through which they maintain tolerance to self-antigens and prevent overactive immune reactions.

Pathways

CTLA-4 involves itself in the adaptive immune response pathways specifically the regulation of T-cell activity. CTLA-4's interaction with CD80/CD86 modulates pathways associated with TCR (T-cell receptor) signaling. When CTLA-4 binds its ligands it recruits phosphatases such as SHP-2 that dephosphorylate signaling proteins leading to the downregulation of T-cell interaction. This pathway interaction exemplifies how CTLA-4 acts in concert with proteins like CD28 to finely tune immune responses.

CTLA-4 plays a role in autoimmune diseases and cancer. The dysregulation of CTLA-4 function can lead to autoimmune disorders where the immune system attacks the body's own tissue. In cancer tumor cells may manipulate CTLA-4 pathways to evade immune surveillance. Targeted therapies like anti-CTLA-4 antibodies such as ipilimumab have been developed to block CTLA-4 aiming to enhance the immune system’s ability to attack cancer cells. These treatments highlight the role of CTLA-4 in balancing immune activity and the potential to alter this balance for therapeutic benefit.
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产品实验方案

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靶点信息

Inhibitory receptor acting as a major negative regulator of T-cell responses (PubMed : 11279501, PubMed : 11279502, PubMed : 16551244, PubMed : 1714933, PubMed : 18641304, PubMed : 28484017). Acts as a decoy receptor : the affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28 (PubMed : 11279501, PubMed : 11279502, PubMed : 16551244, PubMed : 1714933, PubMed : 28484017).
See full target information CTLA4
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 12:17648-17659 PubMed37501393

2023

Serum soluble immune checkpoint levels predict cervical lymph node metastasis of differentiated thyroid carcinoma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Shao,Xinru Gui,Yuxin Wang,Lei Sheng,Dong Sun,Qingdong Zeng,Huayang Wang
View all publications
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websiteProtocolBooklet
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