人CTHRC1 ELISA试剂盒(ab274399)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 27.3 pg/ml
- Range: 93.75 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人CTHRC1 ELISA试剂盒
参阅全部 CTHRC1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Supernatant 8 2.55% 批次间 样品 n Mean SD CV% Supernatant 3 2.88% -
样品类型
Cell culture supernatant, Serum, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
27.3 pg/ml -
范围
93.75 pg/ml - 6000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 100 99% - 101% Serum 97 91% - 101% Cit plasma 100 95% - 103% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human CTHRC1 ELISA kit (ab274399) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CTHRC1 protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CTHRC1 with 27.3 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Collagen triple helix repeat containing protein 1 (CTHRC1) is a 30 kDa secreted glycoprotein encoded by the CTHRC1 gene. CTHRC1 is known to regulate extracellular collagen deposition and partake in vascular remodeling of arterial injury as a function of cellular response through the TGF-β and Wnt pathways. Moreover, mutation of the gene has been associated with carcinogenesis in a variety of cell and tissues, most notably esophageal adenocarcinoma and Barrett’s esophagus. Furthermore, recent studies have demonstrated CTHRC1 as a marker of the severity of the disease progression in the dystrophic muscles.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 1 x 384 tests 10X Human CTHRC1 Capture Antibody 1 x 600µl 1 x 600µl 10X Human CTHRC1 Detector Antibody 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 1 unit Antibody Diluent CPI2 1 x 6ml 1 x 6ml Human CTHRC1 Lyophilized Recombinant Protein 2 vials 2 vials Plate Seal 1 unit 1 unit Sample Diluent NS (ab193972) 1 x 50ml 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 0 x 0 unit Stop Solution 1 x 12ml 2 x 12ml TMB Development Solution 1 x 12ml 2 x 12ml -
研究领域
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功能
May act as a negative regulator of collagen matrix deposition. -
组织特异性
Isoform 1 is expressed in calcified atherosclerotic plaque and chondrocyte-like cells. -
序列相似性
Contains 1 collagen-like domain. -
翻译后修饰
N-glycosylated. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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别名
- Collagen triple helix repeat containing 1
- Collagen triple helix repeat containing protein 1
- Collagen triple helix repeat-containing protein 1
see all -
数据库链接
- Entrez Gene: 115908 Human
- Omim: 610635 Human
- SwissProt: Q96CG8 Human
- Unigene: 405614 Human
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab274399于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Sandwich ELISA - Human CTHRC1 ELISA Kit (ab274399)Interpolated concentration of native CTHRC1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Sandwich ELISA - Human CTHRC1 ELISA Kit (ab274399)Example of human CTHRC1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed. -
Example of human CTHRC1 standard curve in Sample Diluent NS.The CTHRC1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CTHRC1 in human serum, plasma and cell culture supernatant samples.The concentrations of CTHRC1 were measured in duplicates, interpolated from the CTHRC1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, and SK-MEL28 cell culture supernatant 4%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CTHRC1 concentration was determined to be 9,486 pg/mL in serum, 10,463 pg/mL in plasma (citrate) and 140,962 pg/mL in SK-MEL28 cell culture supernatant.
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Sandwich ELISA - Human CTHRC1 ELISA Kit (ab274399)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Sandwich ELISA - Human CTHRC1 ELISA Kit (ab274399)To learn more about the advantages of recombinant antibodies see here. -
Sandwich ELISA - Human CTHRC1 ELISA Kit (ab274399)To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab274399 被引用在 1 文献中.
- Baiken Y et al. Elevated Levels of Plasma Collagen Triple Helix Repeat Containing 1 (CTHRC1) Are Strongly Associated with eGFR and Albuminuria in Chronic Kidney Disease. Medicina (Kaunas) 59:N/A (2023). PubMed: 37109608