人CSPG4 ELISA试剂盒(NG2/MCSP)

产品详情

Human CSPG4 ELISA Kit (NG2/MCSP) (ab267807) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CSPG4 (NG2/MCSP) protein in serum, cit plasma, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CSPG4 (NG2/MCSP) with 25.5 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Chondroitin sulfate proteoglycan 4 (CSPG4), also known as NG2 or MCSP, is a multifunctional cell surface type I transmembrane protein that is covalently modified with chondroitin sulfate glycosaminoglycan. CSPG4 has been observed to promote epidermal stem cell patterned clustering and used as a cell surface marker for melanoma and glial precursor cells. Studies have associate CSPG4 as important role in facilitating the growth and survival of malignant melanoma as well as migration, invasion, metastasis, and chemoresistance.