人Complement C7 ELISA试剂盒(ab125964)
Key features and details
- Sensitivity: 0.18 ng/ml
- Range: 0.313 ng/ml - 20 ng/ml
- Sample type: Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Complement C7 ELISA试剂盒
参阅全部 C7 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 4% 批次间 样品 n Mean SD CV% Overall 10.3% -
样品类型
Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
检测类型
Sandwich (quantitative) -
灵敏度
0.18 ng/ml -
范围
0.313 ng/ml - 20 ng/ml -
回收率
97 %
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检测时间
4h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Complement C7 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C7 in Human plasma, serum, urine, milk, saliva, and CSF samples.
A Complement C7 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C7 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C7 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Complement C7 Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Complement C7 Microplate (12 x 8 well strips) 1 unit Complement C7 Standard (Lyophilized) 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor. -
疾病相关
Defects in C7 are a cause of complement component 7 deficiency (C7D) [MIM:610102]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. -
序列相似性
Belongs to the complement C6/C7/C8/C9 family.
Contains 1 EGF-like domain.
Contains 1 LDL-receptor class A domain.
Contains 1 MACPF domain.
Contains 2 Sushi (CCP/SCR) domains.
Contains 2 TSP type-1 domains. -
翻译后修饰
C7 has 28 disulfide bridges. -
细胞定位
Secreted. - Information by UniProt
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别名
- C7
- CO7_HUMAN
- complement component 7
- Complement component C7
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数据库链接
- Entrez Gene: 730 Human
- Omim: 217070 Human
- SwissProt: P10643 Human
- Unigene: 78065 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125964于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab125964 被引用在 3 文献中.
- Nelke C et al. Eculizumab treatment alters the proteometabolome beyond the inhibition of complement. JCI Insight 8:N/A (2023). PubMed: 37227781
- Abdel-Tawab MS et al. Evaluation of Serum and Gene Expression of Galectin-4, Interleukin-27, and Complement-7 in Hepatitis C Virus-Infected Egyptian Patients. Biomed Res Int 2020:8879758 (2020). PubMed: 33381596
- Liang Y et al. Coagulation cascade and complement system in systemic lupus erythematosus. Oncotarget 9:14862-14881 (2018). PubMed: 29599912