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AB108824

人Complement C4 ELISA试剂盒

Human Complement C4 ELISA Kit

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(1 Review)

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(9 Publications)

Human Complement C4 ELISA Kit is a competitive ELISA designed to quantify Human Complement C4 with a sensitivity of 0.5 µg/mL

- Colorimetric competitive ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.625 - 10 µg/ml

查看别名

CO4, CPAMD2, C4A, Complement C4-A, Acidic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2, CO4, CPAMD3, C4B_2, C4B, Complement C4-B, Basic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3

2 Images
Competitive ELISA - Human Complement C4 ELISA Kit (AB108824)
  • cELISA

Lab

Competitive ELISA - Human Complement C4 ELISA Kit (AB108824)

Standard curve : mean of duplicates (+/-SD)

Competitive ELISA - Human Complement C4 ELISA Kit (AB108824)
  • cELISA

Lab

Competitive ELISA - Human Complement C4 ELISA Kit (AB108824)

Complement C4 measured in biological fluids showing quantity (microgram) per mL of tested sample

关键信息

检测方法

Colorimetric

样品类型

Plasma, Serum

反应种属

Human

检测类型

Competitive

灵敏度

= 0.5 µg/mL

范围

0.625 - 10 µg/mL

检测时间

3h

检测平台

Microplate

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搭配使用产品

Assay Kits

AB108822

Human Complement C3 ELISA Kit

Sandwich ELISA - Human Complement C3 ELISA Kit (AB108822)

  • 5
  • 2
Assay Kits

AB263884

Mouse C3 ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse C3 ELISA Kit (AB263884)

  • 0
  • 0
Assay Kits

AB264609

Mouse Complement C5 ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Complement C5 ELISA Kit (AB264609)

  • 0
  • 0
Assay Kits

AB315320

Human C4a anaphylatoxin ELISA Kit

SimpleStep

Sandwich ELISA - Human C4a anaphylatoxin ELISA Kit (AB315320)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Complement C4 Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab108834) is designed for the quantitative measurement of Complement C4 levels in plasma and serum.

A Complement C4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Complement C4 is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Complement C4 captured in plate.

Reactivity with C4 cleavage products (C4a, C4b, C4d) has not been determined.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108824-1x96Tests", "assetComponentDetails": [ { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108824-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108824-CMP01", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108824-CMP08", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108824-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108824-CMP06", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108824-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Complement C4 Standard", "number":"AB108824-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Complement C4 Microplate (12 x 8 well strips)", "number":"AB108824-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"2X Biotinylated Human Complement C4 (Lyophilized)", "number":"AB108824-CMP03", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
Multi
储存信息
Please refer to protocols
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Complement component 4 (C4) is a significant protein in the complement system also known as C4 complement. C4 has a molecular mass of approximately 206 kDa and mainly expresses in the liver. It is present in the blood plasma. C4 plays an important role in the immunological response and activates classical and lectin complement pathways. It serves a mechanical function by binding to pathogen surfaces and facilitating the cleavage that leads to the downstream activation of the complement cascade. There's a C4 ELISA test that allows for quantifying C4 levels in serum which is important for clinical diagnostics.
Biological function summary

Complement C4 participates in the immune defense by marking pathogens for destruction and enhancing phagocytosis. C4 is a part of a complex with other complement proteins like C3 and C1 to initiate the formation of the C3 convertase enzyme. This action amplifies the immune response leading to the clearance of microorganisms and apoptotic cells. C4 also plays a role in regulating the inflammation process maintaining homeostasis in the immune system.

Pathways

C4 fits into the complement activation pathway specifically the classical and lectin pathways. These pathways are important for innate immunity and connect with components like complement C3 and complement C1. Through the classical pathway C4 gets activated after binding to antibodies that have recognized antigens leading to a chain reaction that strengthens the immune response. The lectin pathway also requires C4 activation through different stimuli solidifying C4's central role in immune system signaling networks.

Abnormal C4 activity associates with autoimmune diseases like systemic lupus erythematosus (SLE) and rheumatoid arthritis. Low levels of complement C4 in serum can indicate these autoimmune conditions where the immune system mistakenly attacks its own tissues. C4 also connects to other proteins like complement component C1q in the context of SLE. C4 deficiencies or abnormalities may contribute to the development or exacerbation of these diseases highlighting its importance in disease pathogenesis and highlighting its value as a target for diagnostic tests such as C4 ELISA or serum complement tests.
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产品实验方案

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靶点信息

Precursor of non-enzymatic components of the classical, lectin and GZMK complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system.. Complement C4b-A. Non-enzymatic component of C3 and C5 convertases (PubMed : 8538770). Generated following cleavage by complement proteases (C1S, MASP2 or GZMK, depending on the complement pathway), it covalently attaches to the surface of pathogens, where it acts as an opsonin that marks the surface of antigens for removal (PubMed : 27738201, PubMed : 8538770). It then recruits the serine protease complement C2b to form the C3 and C5 convertases, which cleave and activate C3 and C5, respectively, the next components of the complement pathways (PubMed : 12878586, PubMed : 18204047, PubMed : 2387864, PubMed : 6906228). Complement C4b-A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while complement C4b-B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens (PubMed : 8538770).. C4a anaphylatoxin. Putative humoral mediator released following cleavage by complement proteases (C1S, MASP2 or GZMK, depending on the complement pathway) (PubMed : 6167582). While it is strongly similar to anaphylatoxins, its role is unclear (PubMed : 25659340). Was reported to act as a mediator of local inflammatory process; however these effects were probably due to contamination with C3a and/C5a anaphylatoxins in biological assays (PubMed : 25659340).
See full target information C4A

其他靶点

C4B
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文献 (9)

Recent publications for all applications. Explore the full list and refine your search

Developmental neuroscience 45:349-360 PubMed37734326

2023

Prospects and Pitfalls of Plasma Complement C4 in Schizophrenia: Building a Better Biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Emily G Severance,Emese Prandovszky,Shuojia Yang,Flora Leister,Ashley Lea,Ching-Lien Wu,Ryad Tamouza,Marion Leboyer,Faith Dickerson,Robert H Yolken

Frontiers in immunology 11:582737 PubMed33133102

2020

Plasma C4d Correlates With C4d Deposition in Kidneys and With Treatment Response in Lupus Nephritis Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Myriam Martin,Rebecca Trattner,Sara C Nilsson,Albin Björk,Agneta Zickert,Anna M Blom,Iva Gunnarsson

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 46:1140-1144 PubMed32961544

2020

Complement component C4 levels in the cerebrospinal fluid and plasma of patients with schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Juan A Gallego,Emily A Blanco,Christopher Morell,Todd Lencz,Anil K Malhotra

The Journal of clinical investigation 130:5909-5923 PubMed32730229

2020

Complement activation on endothelium initiates antibody-mediated acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Simon J Cleary,Nicholas Kwaan,Jennifer J Tian,Daniel R Calabrese,Beñat Mallavia,Mélia Magnen,John R Greenland,Anatoly Urisman,Jonathan P Singer,Steven R Hays,Jasleen Kukreja,Ariel M Hay,Heather L Howie,Pearl Toy,Clifford A Lowell,Craig N Morrell,James C Zimring,Mark R Looney

Scientific reports 10:6236 PubMed32277232

2020

Analysis of the receptor BCMA as a biomarker in systemic lupus erythematosus patients.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Celeste Salazar-Camarena,Claudia Azucena Palafox-Sánchez,Alvaro Cruz,Miguel Marín-Rosales,José Francisco Muñoz-Valle

Journal of cellular biochemistry 121:111-124 PubMed31190349

2019

MicroRNA-194 reduces inflammatory response and human dermal microvascular endothelial cells permeability through suppression of TGF-β/SMAD pathway by inhibiting THBS1 in chronic idiopathic urticaria.

Applications

Unspecified application

Species

Unspecified reactive species

Shengming Qu,Lei Yang,Zhe Liu

Molecular neuropsychiatry 5:52-59 PubMed31019918

2019

X-Aptamer Technology Identifies C4A and ApoB in Blood as Potential Markers for Schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Consuelo Walss-Bass,Ganesh L R Lokesh,Elena Dyukova,David G Gorenstein,David L Roberts,Dawn Velligan,David E Volk

Experimental and therapeutic medicine 15:585-591 PubMed29387207

2018

Toll-like receptor-9 is involved in the development of B cell stimulating factor-induced systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Feng Zhan,Xiao Zhang,Shudian Lin

Investigative ophthalmology & visual science 55:4776-89 PubMed24854857

2014

Associations between abnormal rod-mediated dark adaptation and health and functioning in older adults with normal macular health.

Applications

ELISA

Species

Human

Cynthia Owsley,Carrie Huisingh,Gregory R Jackson,Christine A Curcio,Alexander J Szalai,Nassrin Dashti,Mark Clark,Kia Rookard,Mark A McCrory,Tyler T Wright,Michael A Callahan,Lanning B Kline,C Douglas Witherspoon,Gerald McGwin
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