人Complement C4-Binding蛋白ELISA试剂盒
Human Complement C4-Binding Protein ELISA Kit
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(2 Publications)
Human Complement C4-Binding Protein ELISA Kit is a Sandwich ELISA for the measurement of Human Complement C4-Binding Protein in Human in Biofluids samples.
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C4b-binding protein beta chain, C4BPB
- sELISA
Supplier Data
Sandwich ELISA - Human Complement C4-Binding Protein ELISA Kit (AB222866)
Example of human Complement C4-Binding Protein standard curve.
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产品详情
ab222866 Human Complement C4-Binding Protein ELISA Kit is designed for the quantitative measurement of Complement C4-Binding Protein in plasma, serum, urine, milk, saliva, cerebrospinal fluid (CSF), and cell culture samples.
The kit employs a quantitative sandwich enzyme immunoassay technique that measures human Complement C4-Binding Protein (C4BP) in less than 4 hours. A polyclonal antibody specific for human complement C4BP has been pre-coated onto a 96-well microplate with removable strips. Complement C4BP in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for complement C4BP, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Complement component C4-binding protein (C4BP) regulates the complement system by accelerating the decay of the complement component C3 convertase and by acting as a cofactor to the serine protease factor I in the degradation of C4b. C4BP is a high molecular mass (570 kDa) glycoprotein and is present in plasma in various isoforms with different alpha beta composition. The major form of C4BP is composed of seven identical 70-kDa alpha chains, each containing a binding site for the complement protein C4b, and a unique 45 kDa beta chain which contains a binding site for the vitamin K-dependent protein S. C4BP was overexpressed in the synovial membranes of patients with rheumatoid arthritis. It was detected in amyloid-beta plaques and on apoptotic cells.
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
C4 binding protein plays a defensive role in the immune system by regulating complement activation. It is part of a larger complex that controls the complement pathway which is integral to immune surveillance and removing pathogens. C4BPB functions primarily by binding to complement C4 preventing the formation of the C3 convertase which is an enzyme that amplifies the complement response. This binding action helps in stabilizing the protein C4 and complement C4 serum to avoid unnecessary immune reactions.
Pathways
The complement system pathway prominently features C4 binding protein. In the classical complement activation pathway C4BPB works closely with other proteins such as C3 and C4 to ensure regulatory balance. Its inhibitory action on the complement C4 pathway provides a safeguard against damage to host tissues during immune responses. The interplay between C4BPB and protein C4 offers a protective mechanism reducing excessive inflammation which can lead to tissue injury.
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文献 (2)
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Journal of clinical immunology 41:1607-1620 PubMed34232441
2021
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Frontiers in immunology 12:668725 PubMed34276659
2021
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