人Clusterin ELISA试剂盒(ab174447)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 22.5 pg/ml
- Range: 234.4 pg/ml - 15000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human, Rhesus monkey
概述
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产品名称
人Clusterin ELISA试剂盒
参阅全部 Clusterin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 9 2.7% 批次间 样品 n Mean SD CV% Serum 3 4.9% -
样品类型
Cell culture supernatant, Saliva, Milk, Urine, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
22.5 pg/ml -
范围
234.4 pg/ml - 15000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 103 100% - 106% Saliva 100 99% - 100% Milk 99 97% - 101% Urine 102 101% - 102% Serum 101 98% - 103% Cell culture extracts 105 96% - 112% Hep Plasma 102 101% - 103% EDTA Plasma 101 99% - 102% Cit plasma 107 106% - 109% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human, Rhesus monkey
不与反应: Cow -
产品概述
Human Clusterin ELISA kit (ab174447) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Clusterin protein in human serum, plasma, urine, saliva, milk, cell culture supernatant, and cell extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Clusterin with 36.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits. -
说明
Clusterin (CLU) is composed of an antiparallel, disulfide-linked heterodimer of an alpha chain and a beta chain that self-associate and can form higher oligomers. It is ubiquitously expressed in various tissues. Clusterin functions as an extracellular chaperone that prevents aggregation of nonnative proteins. It does not require ATP and does not refold proteins by itself. Clusterin does so by maintaining partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Clusterin prevents stress-induced aggregation of blood plasma proteins by inhibiting the formation of amyloid fibrils through APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). It can bind to cell surface receptors and trigger internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human Clusterin Capture Antibody 1 x 600µl 10X Human Clusterin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human Clusterin Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. -
组织特异性
Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung. -
序列相似性
Belongs to the clusterin family. -
翻译后修饰
Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
Polyubiquitinated, leading to proteasomal degradation.
Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate. -
细胞定位
Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis. - Information by UniProt
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别名
- 40
- AAG 4
- AAG4
see all -
数据库链接
- Entrez Gene: 1191 Human
- Omim: 185430 Human
- SwissProt: P10909 Human
- Unigene: 436657 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab174447于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Example of human Clusterin standard curve in Sample Diluent NS.
The Clusterin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Clusterin standard curve in 1X Cell Extraction Buffer PTR.The Clusterin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Clusterin in human serum and plasma samples.The concentrations of Clusterin were measured in duplicates, interpolated from the Clusterin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:80,000, plasma (heparin) 1:40,000, plasma (EDTA) 1:40,000, and plasma (citrate) 1:20,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Clusterin concentration in the neat samples was determined to be 174 µg/mL in serum, 123 µg/mL in plasma (heparin), 158 µg/mL in plasma (EDTA), and 206 µg/mL in plasma (citrate).
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Interpolated concentrations of native Clusterin in human urine, saliva, and milk.The concentrations of Clusterin were measured in duplicates, interpolated from the Clusterin standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 1:32, saliva 1:80, and milk 1:3,200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Clusterin concentration in the neat samples was determined to be 157 ng/mL in urine, 246 ng/mL in saliva, and 20,323 ng/mL in milk.
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Interpolated concentrations of native Clusterin in human HepG2 cell supernatant, HepG2 cell extract, and MDA-MB-231 cell extract.The concentrations of Clusterin were measured in duplicates, interpolated from the Clusterin standard curves and corrected for sample dilution. Undiluted samples are as follows: HepG2 supernatant 1:12, HepG2 extract 200 µg/mL, and MDA-MB-231 extract 750 µg/mL.
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Interpolated concentrations of native Clusterin in Rhesus Macaque plasma (EDTA).The concentrations of Clusterin were measured in duplicate, interpolated from the Clusterin standard curve and corrected for sample dilution. Undiluted samples are 1:20,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Clusterin concentration in the neat sample was determined to be 94 µg/mL.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Clusterin concentration was determined to be 150 µg/mL with a range of 92 – 227 µg/mL.
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Human Clusterin Competitor Std Curve ComparisonStandard curve comparison between human Clusterin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Kit Comparison between current and original versionStandard curve comparison between current human Clutsterin SimpleStep ELISA® kit and original human Clusterin SimpleStep ELISA® kit. The current SimpleStep ELISA kit shows increased sensitivity.
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Sandwich ELISA - Human Clusterin ELISA Kit (ab174447)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Sandwich ELISA - Human Clusterin ELISA Kit (ab174447)To learn more about the advantages of recombinant antibodies see here. -
Sandwich ELISA - Human Clusterin ELISA Kit (ab174447)To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab174447 被引用在 9 文献中.
- Oldfield L et al. Blood levels of adiponectin and IL-1Ra distinguish type 3c from type 2 diabetes: Implications for earlier pancreatic cancer detection in new-onset diabetes. EBioMedicine 75:103802 (2022). PubMed: 34990893
- Pecankova K et al. Proteome changes of plasma-derived extracellular vesicles in patients with myelodysplastic syndrome. PLoS One 17:e0262484 (2022). PubMed: 35007303
- Meng Q et al. Study on the Mechanism of Platelet-Released Clusterins Inducing Restenosis after Carotid Endarterectomy by Activating TLR3/NF-κb p65 Signaling Pathway. J Healthc Eng 2022:7631126 (2022). PubMed: 35047156
- Visconti VV et al. Deregulated Clusterin as a Marker of Bone Fragility: New Insights into the Pathophysiology of Osteoporosis. Genes (Basel) 13:N/A (2022). PubMed: 35456459
- Zhang Q et al. Clusterin as a serum biomarker candidate contributes to the lung fibroblasts activation in chronic obstructive pulmonary disease. Chin Med J (Engl) 135:1076-1086 (2022). PubMed: 35191419
- Valencia E et al. Targeted Analysis of Tears Revealed Specific Altered Metal Homeostasis in Age-Related Macular Degeneration. Invest Ophthalmol Vis Sci 63:10 (2022). PubMed: 35426907
- Huang Y et al. Reduced Expression of Antimicrobial Protein Secretory Leukoprotease Inhibitor and Clusterin in Chronic Rhinosinusitis with Nasal Polyps. J Immunol Res 2021:1057186 (2021). PubMed: 33506054
- Whiten DR et al. Single-Molecule Characterization of the Interactions between Extracellular Chaperones and Toxic a-Synuclein Oligomers. Cell Rep 23:3492-3500 (2018). PubMed: 29924993
- Seo JA et al. Circulating ApoJ is closely associated with insulin resistance in human subjects. Metabolism 78:155-166 (2018). PubMed: 28986164