AB174447

人Clusterin ELISA试剂盒

Human Clusterin ELISA Kit

Recombinant
SimpleStep
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(11 Publications)

Human Clusterin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Clusterin with a sensitivity of 22.5 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

APOJ, CLI, KUB1, AAG4, CLU, Clusterin, Aging-associated gene 4 protein, Apolipoprotein J, Complement cytolysis inhibitor, Ku70-binding protein 1, NA1/NA2, Sulfated glycoprotein 2, Testosterone-repressed prostate message 2, Apo-J, SGP-2, TRPM-2

10 Images

ELISA - Human Clusterin ELISA Kit (AB174447)

ELISA

Supplier Data

ELISA - Human Clusterin ELISA Kit (AB174447)

sELISA

Supplier Data

Sandwich ELISA - Human Clusterin ELISA Kit (AB174447)

Example of human Clusterin standard curve in Sample Diluent NS.

The Clusterin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Clusterin ELISA Kit (AB174447)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Clusterin concentration was determined to be 150 μg/mL with a range of 92 – 227 μg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Clusterin ELISA Kit (AB174447)

Interpolated concentrations of native Clusterin in Rhesus Macaque plasma (EDTA).

The concentrations of Clusterin were measured in duplicate, interpolated from the Clusterin standard curve and corrected for sample dilution. Undiluted samples are 1 : 20,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Clusterin concentration in the neat sample was determined to be 94 μg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Clusterin ELISA Kit (AB174447)

Example of human Clusterin standard curve in 1X Cell Extraction Buffer PTR.

The Clusterin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Clusterin ELISA Kit (AB174447)

Interpolated concentrations of native Clusterin in human HepG2 cell supernatant, HepG2 cell extract, and MDA-MB-231 cell extract.

sELISA

Supplier Data

Sandwich ELISA - Human Clusterin ELISA Kit (AB174447)

Human Clusterin Competitor Std Curve Comparison.

Standard curve comparison between human Clusterin SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human Clusterin ELISA Kit (AB174447)

Interpolated concentrations of native Clusterin in human serum and plasma samples.

The concentrations of Clusterin were measured in duplicates, interpolated from the Clusterin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 80,000, plasma (heparin) 1 : 40,000, plasma (EDTA) 1 : 40,000, and plasma (citrate) 1 : 20,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Clusterin concentration in the neat samples was determined to be 174 μg/mL in serum, 123 μg/mL in plasma (heparin), 158 μg/mL in plasma (EDTA), and 206 μg/mL in plasma (citrate).

sELISA

Supplier Data

Sandwich ELISA - Human Clusterin ELISA Kit (AB174447)

Kit Comparison between current and original version.

Standard curve comparison between current human Clutsterin SimpleStep ELISA^® kit and original human Clusterin SimpleStep ELISA^® kit. The current SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human Clusterin ELISA Kit (AB174447)

Interpolated concentrations of native Clusterin in human urine, saliva, and milk.

The concentrations of Clusterin were measured in duplicates, interpolated from the Clusterin standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 32, saliva 1 : 80, and milk 1 : 3,200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Clusterin concentration in the neat samples was determined to be 157 ng/mL in urine, 246 ng/mL in saliva, and 20,323 ng/mL in milk.

关键信息

检测方法

Colorimetric

样品类型

Saliva, Urine, Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

反应种属

Human, Rhesus monkey

检测类型

Sandwich (quantitative)

灵敏度

= 22.5 pg/mL

范围

234.4 - 15000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human Clusterin ELISA Kit ab174447 is a rapid single-wash 90-min ELISA kit to measure Human Clusterin in saliva, urine, cell culture extracts, cell culture supernatant, milk, serum, and plasma. This SimpleStep ELISA® sensitivity is 22.5 pg/mL.

How the assay works

Human Clusterin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and recombinant human Clusterin protein in serum, plasma, urine, saliva, milk, cell culture supernatant, and cell extract samples only. Tissue extract samples have not been tested with this kit. This kit was found to recognize the alpha chain of the Clusterin protein.

Human Clusterin ELISA Kit ab174447 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human Clusterin ELISA Kit ab174447

Human Clusterin ELISA Kit ab174447 has been used to study the role of clusterin in regulating fibroblasts in COPD⁽¹⁾, metal homeostasis in age-related macular degeneration⁽²⁾, and the potential of clusterin as a diagnostic and prognostic marker for osteoporosis⁽³⁾.

References:

⁽¹⁾Rui Zheng et al. 2022, PMID: 35191419,
⁽²⁾Hйctor Gonzбlez-Iglesias et al. 2022, PMID: 35426907,
⁽³⁾Umberto Tarantino et al. 2022, PMID: 35456459

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human/Monkey Clusterin Antibody Pair - BSA and Azide free ab243972
- Anti-Clusterin antibody [EPR20729-240] - BSA and Azide free (Capture) ab244626
- Anti-Clusterin antibody [EPR20729-41] - BSA and Azide free (Detector) ab244885

Related and recommended products

Human Clusterin ELISA Kit ab174447 is commonly used to study metabolism and neurodegenerative diseases.

Other kits to measure lipid metabolism and neurodegeneration include:

- Human Leptin ELISA Kit ab179884
- Human Apo E ELISA Kit ab233623
- Human TREM2 ELISA Kit ab224881

Clusterin (CLU) is composed of an antiparallel, disulfide-linked heterodimer of an alpha chain and a beta chain that self-associate and can form higher oligomers. It is ubiquitously expressed in various tissues. Clusterin functions as an extracellular chaperone that prevents aggregation of nonnative proteins. It does not require ATP and does not refold proteins by itself. Clusterin does so by maintaining partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Clusterin prevents stress-induced aggregation of blood plasma proteins by inhibiting the formation of amyloid fibrils through APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). It can bind to cell surface receptors and trigger internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]

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回收率

[ { "sample": "Cell culture supernatant", "range": "100 - 106 %", "average": "= 103" }, { "sample": "Serum", "range": "98 - 103 %", "average": "= 101" }, { "sample": "Milk", "range": "97 - 101 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "99 - 102 %", "average": "= 101" }, { "sample": "Cell culture extracts", "range": "96 - 112 %", "average": "= 105" }, { "sample": "Urine", "range": "101 - 102 %", "average": "= 102" }, { "sample": "Saliva", "range": "99 - 100 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "101 - 103 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "106 - 109 %", "average": "= 107" } ]

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规格

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Clusterin also known as Apolipoprotein J (ApoJ) is a glycoprotein with a mass of approximately 75-80 kDa. Scientists find clusterin expressed widely in the human body including the brain kidney and reproductive organs. This protein exists in two isoforms a secreted form and a nuclear form each with different locations and functions within the cells.

Biological function summary

Clusterin plays multiple roles particularly in lipid transport apoptosis and cell-cell interactions. It does not form part of a larger protein complex but it acts as a molecular chaperone that helps in folding and clearance of damaged proteins. This capability allows clusterin to protect cells against stress conditions such as oxidative stress by preventing abnormal protein aggregation.

Pathways

Clusterin is involved in pathways related to cell survival and lipid metabolism. It plays a role in the insulin signaling pathway and interacts with proteins like Heat Shock Protein 70 (HSP70) to assist in protein folding and in the activation of apoptotic pathways when needed. Clusterin's function in these processes helps in maintaining cellular homeostasis.

Scientists associate clusterin with Alzheimer’s disease and cancer progression. Elevated levels of clusterin have been observed in Alzheimer's patients suggesting a role in amyloid-beta plaque formation. In cancer clusterin expression influences tumor progression and resistance to therapies. Its interaction with other proteins such as Bax modulates the apoptotic pathways impacting cancer cell survival and chemotherapy resistance.

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产品实验方案

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靶点信息

Isoform 1. Functions as extracellular chaperone that prevents aggregation of non native proteins (PubMed : 11123922, PubMed : 19535339). Prevents stress-induced aggregation of blood plasma proteins (PubMed : 11123922, PubMed : 12176985, PubMed : 17260971, PubMed : 19996109). Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro) (PubMed : 12047389, PubMed : 17407782, PubMed : 17412999). Does not require ATP (PubMed : 11123922). Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70 (PubMed : 11123922). Does not refold proteins by itself (PubMed : 11123922). Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation (PubMed : 21505792). Protects cells against apoptosis and against cytolysis by complement (PubMed : 2780565). Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 20068069). Promotes proteasomal degradation of COMMD1 and IKBKB (PubMed : 20068069). Modulates NF-kappa-B transcriptional activity (PubMed : 12882985). A mitochondrial form suppresses BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis (PubMed : 16113678, PubMed : 17689225). Plays a role in the regulation of cell proliferation (PubMed : 19137541). An intracellular form suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5 (PubMed : 22689054). Secreted form does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity (PubMed : 24073260). Secreted form act as an important modulator during neuronal differentiation through interaction with STMN3 (By similarity). Plays a role in the clearance of immune complexes that arise during cell injury (By similarity).. Isoform 6. Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity.. Isoform 4. Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity (PubMed : 24073260). Promotes cell death through interaction with BCL2L1 that releases and activates BAX (PubMed : 21567405).

See full target information CLU

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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

Clinical proteomics 21:61 PubMed39487396

2024

Serum proteomics for the identification of biomarkers to flag predilection of COVID19 patients to various organ morbidities.

Applications

Unspecified application

Species

Unspecified reactive species

Madhan Vishal Rajan,Vipra Sharma,Neelam Upadhyay,Ananya Murali,Sabyasachi Bandyopadhyay,Gururao Hariprasad

Antimicrobial agents and chemotherapy 68:e0021924 PubMed39225483

2024

Evaluating nephrotoxicity reduction in a novel polymyxin B formulation: insights from a 3D kidney-on-a-chip model.

Applications

Unspecified application

Species

Unspecified reactive species

Anurag Payasi,Manoj Kumar Yadav,Saransh Chaudhary,Anmol Aggarwal

Chinese medical journal 135:1076-1086 PubMed35191419

2022

Clusterin as a serum biomarker candidate contributes to the lung fibroblasts activation in chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Zhang,Yuanyi Yue,Rui Zheng

Investigative ophthalmology & visual science 63:10 PubMed35426907

2022

Targeted Analysis of Tears Revealed Specific Altered Metal Homeostasis in Age-Related Macular Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Valencia,Montserrat García,Beatriz Fernández-Vega,Rosario Pereiro,Lara Lobo,Héctor González-Iglesias

Genes 13: PubMed35456459

2022

Deregulated Clusterin as a Marker of Bone Fragility: New Insights into the Pathophysiology of Osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Virginia Veronica Visconti,Chiara Greggi,Ida Cariati,Beatrice Gasperini,Ambra Mastrogregori,Annalisa Botta,Umberto Tarantino

Journal of healthcare engineering 2022:7631126 PubMed35047156

2022

Study on the Mechanism of Platelet-Released Clusterins Inducing Restenosis after Carotid Endarterectomy by Activating TLR3/NF-b 65 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qingyu Meng,Xichun Li,Mingyu Zhao,Shusen Lin,Xiangwen Yu,Guanglong Dong

PloS one 17:e0262484 PubMed35007303

2022

Proteome changes of plasma-derived extracellular vesicles in patients with myelodysplastic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Klara Pecankova,Pavla Pecherkova,Zdenka Gasova,Zofie Sovova,Tomas Riedel,Eliézer Jäger,Jaroslav Cermak,Pavel Majek

EBioMedicine 75:103802 PubMed34990893

2022

Blood levels of adiponectin and IL-1Ra distinguish type 3c from type 2 diabetes: Implications for earlier pancreatic cancer detection in new-onset diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Lucy Oldfield,Anthony Evans,Rohith Gopala Rao,Claire Jenkinson,Tejpal Purewal,Eftychia E Psarelli,Usha Menon,John F Timms,Stephen P Pereira,Paula Ghaneh,William Greenhalf,Christopher Halloran,Eithne Costello

Journal of immunology research 2021:1057186 PubMed33506054

2021

Reduced Expression of Antimicrobial Protein Secretory Leukoprotease Inhibitor and Clusterin in Chronic Rhinosinusitis with Nasal Polyps.

Applications

Unspecified application

Species

Unspecified reactive species

Yanran Huang,Ming Wang,Yu Hong,Xiangting Bu,Ge Luan,Yang Wang,Ying Li,Hongfei Lou,Chengshuo Wang,Luo Zhang

Cell reports 23:3492-3500 PubMed29924993

2018

Single-Molecule Characterization of the Interactions between Extracellular Chaperones and Toxic α-Synuclein Oligomers.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel R Whiten,Dezerae Cox,Mathew H Horrocks,Christopher G Taylor,Suman De,Patrick Flagmeier,Laura Tosatto,Janet R Kumita,Heath Ecroyd,Christopher M Dobson,David Klenerman,Mark R Wilson

View all publications

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websiteProtocolBooklet

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人Clusterin ELISA试剂盒

Human Clusterin ELISA Kit

ELISA - Human Clusterin ELISA Kit (AB174447)

Sandwich ELISA - Human Clusterin ELISA Kit (AB174447)

Sandwich ELISA - Human Clusterin ELISA Kit (AB174447)

Sandwich ELISA - Human Clusterin ELISA Kit (AB174447)

Sandwich ELISA - Human Clusterin ELISA Kit (AB174447)

Sandwich ELISA - Human Clusterin ELISA Kit (AB174447)

Sandwich ELISA - Human Clusterin ELISA Kit (AB174447)

Sandwich ELISA - Human Clusterin ELISA Kit (AB174447)

Sandwich ELISA - Human Clusterin ELISA Kit (AB174447)

Sandwich ELISA - Human Clusterin ELISA Kit (AB174447)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台