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AB314850

人CFHR2 ELISA试剂盒

Human CFHR2 ELISA Kit

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Human CFHR2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CFHR2 with a sensitivity of 5.217 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

CFHL2, FHR2, HFL3, CFHR2, Complement factor H-related protein 2, FHR-2, DDESK59, H factor-like 3, H factor-like protein 2

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Sandwich ELISA - Human CFHR2 ELISA Kit (AB314850)
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Supplier Data

Sandwich ELISA - Human CFHR2 ELISA Kit (AB314850)

Interpolated concentrations of human CFHR2 in serum, plasma (EDTA), plasma (Citrate), and plasma (Heparin)

Interpolated concentration of native CFHR2 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 8,000, plasma (EDTA) 1 : 4,000, plasma (Citrate) 1 : 4,000, and plasma (Heparin) 1 : 4,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human CFHR2 ELISA Kit (AB314850)
  • sELISA

Supplier Data

Sandwich ELISA - Human CFHR2 ELISA Kit (AB314850)

Example of human CFHR2 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma

反应种属

Human

检测类型

Sandwich

灵敏度

= 5.217 pg/mL

范围

18.75 - 1200 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human CFHR2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CFHR2 protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma. Quantitate Human CFHR2 with 5.217 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.45" } ]
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回收率

[ { "sample": "Serum", "range": "95 - 107 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "99 - 108 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "94 - 109 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "96 - 104 %", "average": "= 100" } ]
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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CFHL2 also known as Complement Factor H-Related 2 is a small protein with a mass of approximately 29 kDa. It belongs to the family of complement factor H-related proteins. This protein is expressed predominantly in the liver and also in several other tissues including the blood. Its mechanical function involves binding to certain molecules in the extracellular matrix and surfaces which affects the regulation of the complement system.
Biological function summary

CFHL2 plays a role in the immune system by participating in the regulation of the complement activation. It does not operate alone but is part of a group of proteins that modulate the complement system which helps clear pathogens from an organism. CFHL2 can act by competing with complement factor H potentially altering the activity of the complement system and influencing immune responses.

Pathways

CFHL2 interacts with the alternative pathway of the complement system. This pathway is one of three that facilitate the complement system an important part of innate immunity. Its interaction with complement factor H influences the level of activity within this pathway impacting how the body responds to pathogens. CFHL2 in this way indirectly connects to other proteins regulating the immune response.

CFHL2 has been associated with age-related macular degeneration and atypical hemolytic uremic syndrome. In these conditions an imbalance in the complement system is observed where CFHL2's role in modulating complement factor H comes into play. Such disruption in balance contributes to the pathology of these diseases and implicates related proteins like complement factor H in disease progression.
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产品实验方案

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靶点信息

Involved in complement regulation. The dimerized forms have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH. Can associate with lipoproteins and may play a role in lipid metabolism.
See full target information CFHR2
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websiteProtocolBooklet
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搭配使用产品

Primary Antibodies

AB303639

Anti-CFHR2 antibody [EPR25758-10] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-CFHR2 antibody [EPR25758-10] - BSA and Azide free (Capture) (AB303639)

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