人CDKN2A/p16INK4a ELISA试剂盒

产品详情

Human CDKN2A/p16INK4a ELISA Kit ab227903 is a rapid single-wash 90-min Sandwich ELISA to measure Human CDKN2A/p16INK4a in cell culture extracts, tissue extracts. This SimpleStep sensitivity is 1.3 ng/mL.

How the assay works

Human CDKN2A/p16INK4a SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CDKN2A/p16INK4a ELISA Kit ab227903 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CDKN2A/p16INK4a antibody [EPR20419-75] - BSA and Azide free (Capture) ab244961
- Anti-CDKN2A/p16INK4a antibody [EPR20419-262] - BSA and Azide free (Detector) ab244961

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.