人CD63 ELISA试剂盒(ab275099)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 33 pg/ml
- Range: 46.9 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人CD63 ELISA试剂盒
参阅全部 CD63 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 7 6.4% 批次间 样品 n Mean SD CV% Serum 3 7.7% -
样品类型
Cell culture supernatant, Serum, EDTA Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
33 pg/ml -
范围
46.9 pg/ml - 3000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 97 96% - 98% Serum 97 94% - 101% EDTA Plasma 94 90% - 96% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human CD63 ELISA kit (ab275099) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD63 protein in cell culture supernatant, plasma EDTA, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD63 with 33 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Human CD63 antigen, also referred to as Granulophysin, Lysomal-associated membrane protein 3 (LAMP-3), Tetraspanin-30, and Ocular melanoma-associated antigen, is a membrane bound protein encoded by the gene CD63. CD63 belongs to a group of proteins known as the tetraspanin family, which are characterized by the presence of four hydrophobic domains and are found to mediate signal transduction events for the regulation of cell development, activation, growth, and motility. Deficiency of this protein is associated with Hermansky-Pudlak Syndrome, which is an autosomal disorder.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 1 x 384 tests 10X Human CD63 Capture Antibody 1 x 600µl 1 x 600µl 10X Human CD63 Detector Antibody 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 1 unit Antibody Diluent CPI2 1 x 6ml 1 x 6ml Human CD63 Lyophilized Recombinant Protein 2 vials 2 vials Plate Seal 1 unit 1 unit Sample Diluent NS (ab193972) 1 x 12ml 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 0 x 0 unit Stop Solution 1 x 12ml 2 x 12ml TMB Development Solution 1 x 12ml 2 x 12ml -
研究领域
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功能
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
组织特异性
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
序列相似性
Belongs to the tetraspanin (TM4SF) family. -
细胞定位
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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别名
- Lysosomal associated membrane protein 3
- CD 63
- CD63
see all -
数据库链接
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab275099于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Sandwich ELISA - Human CD63 ELISA Kit (ab275099)Example of human CD63 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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SimpleStep ELISA Protocol DiagramSimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CD63 standard curve in Sample Diluent NS.The CD63 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Serum from eight individual healthy CD63 female donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD63 concentration was determined to be 252 pg/mL with a range of 103 – 408 pg/mL.
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Sandwich ELISA - Human CD63 ELISA Kit (ab275099)To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab275099 被引用在 2 文献中.
- Kashkanova AD et al. Label-free discrimination of extracellular vesicles from large lipoproteins. J Extracell Vesicles 12:e12348 (2023). PubMed: 37489102
- Shi Y et al. Single-cell phenotypic profiling to identify a set of immune cell protein biomarkers for relapsed and refractory diffuse large B cell lymphoma: A single-center study. J Leukoc Biol 112:1633-1648 (2022). PubMed: 36040107