人CD62L ELISA试剂盒(Selectin L) (ab45917)
Key features and details
- Sensitivity: 1 ng/ml
- Range: 1.9 ng/ml - 60 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人CD62L ELISA试剂盒(Selectin L)
参阅全部 CD62L 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Sample A 15 28ng/ml 1.3 4.6% Sample B 19 9ng/ml 0.5 5.5% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 1 ng/ml -
范围
1.9 ng/ml - 60 ng/ml -
检测时间
1h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s CD62L (Selectin L) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD62L in Human serum, plasma, buffered solutions and cell culture medium.
A monoclonal antibody specific for CD62L has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD62L concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD62L are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD62L present in the samples.
This kit will recognize both endogenous and recombinant Human CD62L.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Biotinylated anti-CD62L antibody Red 1 x 0.4ml 2 x 0.4ml CD62L Control Silver 2 vials 4 vials CD62L Microplate (12 x 8 well strips) 1 unit 2 units CD62L Standard Yellow 2 vials 4 vials Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 11ml 1 x 23ml Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究领域
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相关性
CD62L is a cell surface component that is a member of a family of adhesion/homing receptors. These play important roles in leukocyte-endothelial cell interactions. CD62L is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins. -
细胞定位
Cell Membrane; single-pass type I membrane protein. -
别名
- A.11
- AI528707
- CD62 antigen ligand
see all -
数据库链接
- Entrez Gene: 6402 Human
- Omim: 153240 Human
- SwissProt: P14151 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab45917于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA |
Use at an assay dependent concentration.
|
说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab45917 被引用在 1 文献中.
- Chen R et al. Differentially expressed RNA from public microarray data identifies serum protein biomarkers for cross-organ transplant rejection and other conditions. PLoS Comput Biol 6: (2010). ELISA ; Human . PubMed: 20885780