人CD59 ELISA试剂盒
Human CD59 ELISA Kit
- Recombinant
- SimpleStep
- 了解详情
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(3 Publications)
Human CD59 ELISA Kit is a single-wash 90-min SimpleStep ELISA used to quantify Human CD59 with a sensitivity of 4.9 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab326396
查看别名
CD59, MIC11, MIN1, MIN2, MIN3, MSK21, CD59 glycoprotein, 1F5 antigen, 20 kDa homologous restriction factor, MAC-inhibitory protein, MEM43 antigen, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin, HRF-20, HRF20, MAC-IP, MACIF, MIRL
- ELISA
Supplier Data
ELISA - Human CD59 ELISA Kit (AB263893)
- sELISA
Supplier Data
Sandwich ELISA - Human CD59 ELISA Kit (AB263893)
Interpolated concentrations of native CD59 in HeLa, HUVEC, and liver cell and tissue culture extracts based on a 62.5 μg/mL, 25 μg/mL, and 31.25 μg/mL extract load, respectively.
The concentrations of CD59 were measured in duplicate and interpolated from the CD59 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD59 concentration was determined to be 2.70 ng/mL in HeLa extract, 2.70 ng/mL in HUVEC extract, and 0.77 ng/mL in liver extract.
- sELISA
Supplier Data
Sandwich ELISA - Human CD59 ELISA Kit (AB263893)
6.25% Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD59 concentration was determined to be 11.88 ng/mL with a range of 9.37 – 18.19 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human CD59 ELISA Kit (AB263893)
Interpolated concentrations of native CD59 in human serum and plasma samples.
The concentrations of CD59 were measured in duplicates, interpolated from the CD59 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (citrate) 12.5%, plasma (EDTA) 12.5%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD59 concentration was determined to be 12.87 ng/mL in serum, 11.50 ng/mL in plasma (citrate), 9.08 ng/mL in plasma (EDTA), and 11.63 ng/mL in plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human CD59 ELISA Kit (AB263893)
Interpolated concentrations of native CD59 in human urine and cell culture supernatant samples.
The concentrations of CD59 were measured in duplicates, interpolated from the CD59 standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 3200 and HUVEC supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD59 concentration was determined to be 1328.22 ng/mL in urine and 8.97 ng/mL in HUVEC supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Human CD59 ELISA Kit (AB263893)
Example of human CD59 standard curve in Sample Diluent NS + 1X Enhancer.
The CD59 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
搭配使用产品
反应性数据
产品详情
Human CD59 ELISA Kit ab263893 is a rapid single-wash 90-min Sandwich ELISA to measure Human CD59 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts, urine. This SimpleStep sensitivity is 4.9 pg/mL.
How the assay works
Human CD59 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human CD59 ELISA Kit ab263893 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-CD59 antibody [EPR22394-28] - BSA and Azide free (Capture) ab267422
- Anti-CD59 antibody [EPR22394-40] - BSA and Azide free (Detector) ab267422
CD59 is a potent inhibitor of the complement membrane attack complex (MAC). It acts by binding to C8 and/or C9 of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. The soluble form from urine retains its specific complement binding activity but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. Glycated CD59 is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. Human CD59 shares 46%, 44%, and 47% sequence homology with mouse, rat, and bovine, respectively.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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性能和储存信息
运输条件
推荐的短期储存条件
推荐的长期储存条件
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
One important feature of CD59 is its role as a glycosylphosphatidylinositol (GPI)-anchored protein involved in safeguarding cells from complement-mediated damage. It does not exist within a larger complex but functions at the cell surface to inhibit the assembly of complement components C5b-9 which form the MAC. This ability to inhibit MAC is essential in maintaining self-tissue from unintended damage during immune responses.
Pathways
CD59 participates in the regulation of the complement cascade specifically within the terminal pathway. The complement system serves as a bridge between innate and adaptive immunity contributing to processes like opsonization and cell lysis. CD59's inhibitory action directly impacts pathways that utilize terminal components such as C5b. Effective CD59 function prevents excessive complement activation ensuring that an immune response does not damage host tissues. It links closely with other complement regulatory proteins like CD55 which also mitigate complement cascade activation.
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文献 (3)
Recent publications for all applications. Explore the full list and refine your search
Molecular therapy. Methods & clinical development 33:101450 PubMed40231247
2025
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International journal of molecular sciences 25: PubMed39518935
2024
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Bioengineering (Basel, Switzerland) 10: PubMed38002385
2023
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