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AB45912

人CD44 ELISA试剂盒

Human CD44 ELISA Kit

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(8 Publications)

Human CD44 ELISA Kit is a sandwich ELISA designed to quantify Human CD44 with a sensitivity of 0.113 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.31 - 10 ng/mL

查看别名

CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I

2 Images
Sandwich ELISA - Human CD44 ELISA Kit (AB45912)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD44 ELISA Kit (AB45912)

Representative Dose Response Curve (Logarithmic) using ab45912.

Sandwich ELISA - Human CD44 ELISA Kit (AB45912)
  • sELISA

Lab

Sandwich ELISA - Human CD44 ELISA Kit (AB45912)

huCD44 measured in biofluids showing quantity (ng) per mL of tested sample

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

< 0.113 ng/mL

范围

0.31 - 10 ng/mL

检测时间

2h 15m

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human CD44 ELISA Kit ab45912 is a sandwich ELISA to measure Human CD44 in serum, plasma, cell culture supernatant with a sensitivity of 0.113 ng/ml.

How the assay works

A monoclonal antibody specific for CD44 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD44 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD44 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD44 present in the samples.

This kit will recognize both endogenous and recombinant Human CD44.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human CD44 ELISA Kit ab45912 protocol summary

1. Add standard or sample to each well used
2. Add prepared Biotinylated labeled detector antibody. Incubate at room temperature
3. Aspirate and wash each well
4. Add prepared Streptavidin-HRP mix to each well. Incubate at room temperature
5. Aspirate and wash each well.
6. Add the TMB Solution to each well until color develops and then add the Stop Solution
7. Immediately begin recording the color development

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精确度

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 50, "mean": "9.95 ng/mL", "standardDeviation": "0.307", "coefficientOfVariability": "3.08" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 50, "mean": "1.07 ng/mL", "standardDeviation": "0.105", "coefficientOfVariability": "9.34" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 8, "mean": "9.85 ng/mL", "standardDeviation": "1.55", "coefficientOfVariability": "1.52" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 8, "mean": "10.5 ng/mL", "standardDeviation": "0.055", "coefficientOfVariability": "4.76" } ]
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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

The protein expressed by gene CD44 functions as a cell-surface receptor involved in cell-cell interactions, cell adhesion, and migration, allowing cells to detect and react to changes in their tissue microenvironment. It is implicated in numerous cellular processes, including the activation, recirculation, and homing of T-lymphocytes, hematopoiesis, inflammation, and the response to bacterial infection. Through its ectodomain, CD44 interacts with extracellular matrix components like hyaluronan, collagen, growth factors, cytokines, and proteases, serving as a platform for signal transduction by forming protein complexes with receptor kinases and membrane proteases via its cytoplasmic domain. Effectors such as PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases, and phospholipase C coordinate signaling pathways that promote calcium mobilization and actin-mediated cytoskeleton reorganization crucial for cell migration and adhesion. This supplementary information is collated from multiple sources and compiled automatically.
See full target information CD44
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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:645 PubMed36746966

2023

Establishment and validation of a plasma oncofetal chondroitin sulfated proteoglycan for pan-cancer detection.

Applications

Unspecified application

Species

Unspecified reactive species

Pei-Fen Zhang,Zi-Yi Wu,Wen-Bin Zhang,Yong-Qiao He,Kexin Chen,Tong-Min Wang,Haixin Li,Hong Zheng,Dan-Hua Li,Da-Wei Yang,Ting Zhou,Chang-Mi Deng,Ying Liao,Wen-Qiong Xue,Lian-Jing Cao,Xi-Zhao Li,Jiang-Bo Zhang,Si-Qi Dong,Fang Wang,Mei-Qi Zheng,Wen-Li Zhang,Jianbing Mu,Wei-Hua Jia

Journal of cellular and molecular medicine 26:2089-2103 PubMed35146909

2022

CD44/ERM/F-actin complex mediates targeted nuclear degranulation and excessive neutrophil extracellular trap formation during sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Yiming Shao,Linbin Li,Lu Liu,Yunxi Yang,Jiamin Huang,Dongdong Ji,Yuying Zhou,Yi Chen,Zhechen Zhu,Bingwei Sun

Zhongguo fei ai za zhi = Chinese journal of lung cancer 24:583-590 PubMed34187156

2021

[Expression of CD44 in Tumor Tissue and Serum of Small Cell Lung Cancer and Its Clinical Prognostic Significance].

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang,Yi Guo,Haifeng Lin,Lina Zhang,Hongmei Zhang,Qunhui Wang,Fanbin Hu,Jie Li,Baolan Li,Tongmei Zhang

Molecules (Basel, Switzerland) 26: PubMed33440679

2021

Zeaxanthin-Rich Extract from Superfood Selectively Modulates the Cellular Adhesion and MAPK Signaling in Melanoma versus Normal Skin Cells In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Cenariu,Eva Fischer-Fodor,Adrian Bogdan Țigu,Andrea Bunea,Piroska Virág,Maria Perde-Schrepler,Vlad-Alexandru Toma,Andrei Mocan,Ioana Berindan-Neagoe,Adela Pintea,Gianina Crișan,Mihai Cenariu,Alma Maniu

Cancer research 74:2630-41 PubMed24618343

2014

CD44v8-10 is a cancer-specific marker for gastric cancer stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Min Lau,Eileen Teng,Hui Shan Chong,Kirsten Anne Pagaduan Lopez,Amy Yuh Ling Tay,Manuel Salto-Tellez,Asim Shabbir,Jimmy Bok Yan So,Shing Leng Chan

Cell death & disease 4:e613 PubMed23640455

2013

A caspase-3 'death-switch' in colorectal cancer cells for induced and synchronous tumor apoptosis in vitro and in vivo facilitates the development of minimally invasive cell death biomarkers.

Applications

ELISA

Species

Human

K L Simpson,C Cawthorne,C Zhou,C L Hodgkinson,M J Walker,F Trapani,M Kadirvel,G Brown,M J Dawson,M MacFarlane,K J Williams,A D Whetton,C Dive

PloS one 7:e37797 PubMed22629457

2012

CCL18 in a multiplex urine-based assay for the detection of bladder cancer.

Applications

ELISA

Species

Human

Virginia Urquidi,Jeongsoon Kim,Myron Chang,Yunfeng Dai,Charles J Rosser,Steve Goodison

PLoS computational biology 6: PubMed20885780

2010

Differentially expressed RNA from public microarray data identifies serum protein biomarkers for cross-organ transplant rejection and other conditions.

Applications

ELISA

Species

Human

Rong Chen,Tara K Sigdel,Li Li,Neeraja Kambham,Joel T Dudley,Szu-Chuan Hsieh,R Bryan Klassen,Amery Chen,Tuyen Caohuu,Alexander A Morgan,Hannah A Valantine,Kiran K Khush,Minnie M Sarwal,Atul J Butte
View all publications
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