AB267801

人CD38 ELISA试剂盒

Human CD38 ELISA Kit

Recombinant
SimpleStep
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Human CD38 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD38 with a sensitivity of 7.44 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

CD38, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, 2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-cyclic-ADP-ribose transferase, ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1, T10, ADPRC 1, cADPR hydrolase 1

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Sandwich ELISA - Human CD38 ELISA Kit (AB267801)

sELISA

Supplier Data

Sandwich ELISA - Human CD38 ELISA Kit (AB267801)

Interpolated concentrations of native CD38 in human Daudi cell culture supernatant sample.

The concentrations of CD38 were measured in duplicates, interpolated from the CD38 standard curves and corrected for sample dilution. Undiluted samples are as follows : Daudi cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD38 concentration was determined to be 1,493 pg/mL in neat Daudi supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human CD38 ELISA Kit (AB267801)

Interpolated concentrations of native CD38 in human serum, plasma-citrate, plasma-EDTA and plasma-heparin samples.

The concentrations of CD38 were measured in duplicates in neat samples and interpolated from the CD38 standard curves. The interpolated values are plotted (mean +/- SD, n=2). The mean CD38 concentration was determined to be 47.6 pg/mL in serum, 35.0 pg/mL in plasma-citrate, 51.4 pg/mL in plasma-EDTA and 39.5 pg/mL in plasma-heparin.

sELISA

Supplier Data

Sandwich ELISA - Human CD38 ELISA Kit (AB267801)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD38 concentration was determined to be 51.8 pg/mL with a range of 36.6 – 82.2 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human CD38 ELISA Kit (AB267801)

Example of human CD38 standard curve in Sample Diluent NS.

The CD38 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human CD38 ELISA Kit (AB267801)

Interpolated concentrations of native CD38 in human Raji and Daudi cells based on a 5 μg/mL extract load.

The concentrations of CD38 were measured in duplicate and interpolated from the CD38 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD38 concentration was determined to be 1,268 pg/mL in Raji extract and 1,323 pg/mL in Daudi extract.

关键信息

检测方法

Colorimetric

样品类型

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma

反应种属

Human

检测类型

Sandwich

灵敏度

= 7.44 pg/mL

范围

31.25 - 2000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human CD38 ELISA Kit (ab267801) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD38 protein in cell culture supernatant, cell lysate, edta plasma, hep plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD38 with 7.44 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CD38, also known as ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (EC:3.2.2.6), is a single-pass type II membrane protein. CD38 catalyzes synthesis of cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. It also has a cyclic ADP-ribose hydrolase activity.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]

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回收率

[ { "sample": "Cell culture supernatant", "range": "98 - 117 %", "average": "= 107" }, { "sample": "Serum", "range": "82 - 88 %", "average": "= 85" }, { "sample": "EDTA Plasma", "range": "76 - 90 %", "average": "= 82" }, { "sample": "Heparin Plasma", "range": "85 - 90 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "90 - 93 %", "average": "= 91" }, { "sample": "Cell Lysate", "range": "75 - 94 %", "average": "= 87" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267801-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB267801-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB267801-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB267801-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB267801-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB267801-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB267801-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB267801-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD38 Capture Antibody", "number":"AB267801-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD38 Lyophilized Recombinant Protein", "number":"AB267801-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD38 Detector Antibody", "number":"AB267801-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB267801-CMP08", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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产品实验方案

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靶点信息

Multifunctional transmembrane glycoprotein able to exert enzymatic activities and also to mobilize calcium, to transduce signals, to adhere to hyaluronan and to other ligands. Synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion (PubMed : 7961800, PubMed : 8253715). Synthesizes the Ca(2+) mobilizer nicotinate-adenine dinucleotide phosphate, NAADP(+), from 2'-phospho-cADPR and nicotinic acid, as well as from NADP(+) and nicotinic acid. At both pH 5.0 and pH 7.4 preferentially transforms 2'-phospho-cADPR into NAADP(+), while preferentially cleaving NADP(+) to cADPR and ADPRP rather than into NADDP(+) (PubMed : 16690024). Has cADPR hydrolase activity (PubMed : 7961800, PubMed : 8253715). Functions also as a receptor that binds the ligand CD31 on endothelial cells, promoting lymphocyte activation, proliferation, and migration across the endothelial barrier (PubMed : 9551996). Involved in the regulation of crucial dendritic cell functions acquired at the mature stage, such as CCL21-driven migration, survival, and Th1-polarizing activity (PubMed : 16293598). In lamina propria T lymphocytes, CD38/CD31 cognate interactions initiate a multistep signaling pathway resulting in activation of LCK anf LAT, followed by cytokine release (PubMed : 11259373).

See full target information CD38

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websiteProtocolBooklet

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Abcam Product Promise

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详情请参阅我们的条款与条件。

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For licensing inquiries, please contact partnerships@abcam.com

人CD38 ELISA试剂盒

Human CD38 ELISA Kit

Sandwich ELISA - Human CD38 ELISA Kit (AB267801)

Sandwich ELISA - Human CD38 ELISA Kit (AB267801)

Sandwich ELISA - Human CD38 ELISA Kit (AB267801)

Sandwich ELISA - Human CD38 ELISA Kit (AB267801)

Sandwich ELISA - Human CD38 ELISA Kit (AB267801)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台