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AB283542

人CD33 ELISA试剂盒

Human CD33 ELISA Kit

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Human CD33 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD33 with a sensitivity of 11.39 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

CD33, SIGLEC3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, Siglec-3

4 Images
ELISA - Human CD33 ELISA Kit (AB283542)
  • ELISA

Supplier Data

ELISA - Human CD33 ELISA Kit (AB283542)
Sandwich ELISA - Human CD33 ELISA Kit (AB283542)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD33 ELISA Kit (AB283542)

Interpolated concentrations of native CD33 in human U937 extract, THP-1 extract, and HL-60 extract based on a 500 μg/mL extract load.

The concentrations of CD33 were measured in duplicate and interpolated from the CD33 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD33 concentration was determined to be 1024.31 pg/mL in U937 extract, 688.05 pg/mL in THP-1 extract and 2363.02 pg/mL in HL-60 extract.

Sandwich ELISA - Human CD33 ELISA Kit (AB283542)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD33 ELISA Kit (AB283542)

Interpolated concentrations of native CD33 in human serum and plasma.

The concentrations of CD33 were measured in duplicates, interpolated from the CD33 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 50%, plasma (EDTA) 50% and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD33 concentration was determined to be 1659.34 pg/mL in serum, 1253.61 pg/mL in plasma (citrate) and 1395.24 pg/mL in plasma (EDTA) and 1288.95 in plasma (heparin).

Sandwich ELISA - Human CD33 ELISA Kit (AB283542)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD33 ELISA Kit (AB283542)

Example of human CD33 standard curve in Sample Diluent NS.

The CD33 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Heparin Plasma, Serum, Cell Lysate, EDTA Plasma

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 11.39 pg/mL

范围

70.31 - 4500 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human CD33 ELISA Kit ab283542 is a rapid single-wash 90-min Sandwich ELISA to measure Human CD33 in cell lysate, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 11.39 pg/mL.

How the assay works

Human CD33 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CD33 ELISA Kit ab283542 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CD33 antibody [EPR23052-20] - BSA and Azide free (Capture) ab281029
- Anti-CD33 antibody [EPR23052-5] - BSA and Azide free (Detector) ab281029

Human CD33 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Human CD33 preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, it may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Human CD33 Induces apoptosis in acute myeloid leukemia (in vitro).

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]
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回收率

[ { "sample": "Serum", "range": "97 - 101 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "90 - 92 %", "average": "= 91" }, { "sample": "Heparin Plasma", "range": "89 - 90 %", "average": "= 89" }, { "sample": "Cell Lysate", "range": "86 - 98 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "83 - 93 %", "average": "= 88" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283542-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB283542-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB283542-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB283542-CMP13", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB283542-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB283542-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB283542-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD33 Lyophilized Recombinant Protein", "number":"AB283542-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB283542-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD33 Capture Antibody", "number":"AB283542-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD33 Detector Antibody", "number":"AB283542-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB283542-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB283542-CMP05", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed : 10611343, PubMed : 11320212, PubMed : 15597323). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed : 7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed : 10887109, PubMed : 28325905). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed : 10206955, PubMed : 10556798, PubMed : 10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed : 10206955, PubMed : 10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed : 15597323).
See full target information CD33
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Abcam Product Promise

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详情请参阅我们的条款与条件。
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