人CD300a ELISA试剂盒(ab317784)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.063 pg/ml
- Range: 15.625 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人CD300a ELISA试剂盒
参阅全部 CD300a 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 8 6.4% 批次间 样品 n Mean SD CV% Serum 3 4.7% -
样品类型
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
4.063 pg/ml -
范围
15.625 pg/ml - 1000 pg/ml -
回收率
98 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 103 99% - 106% Serum 98 88% - 104% Hep Plasma 102 94% - 114% EDTA Plasma 105 101% - 107% Cit plasma 98 91% - 103% -
检测时间
1h 30m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Human CD300a SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD300a protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human CD300a with 4.063 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format. -
经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 1 x 384 tests 10 x 96 tests 10X Human CD300a Capture Antibody 1 x 600µl 1 x 600µl 10 x 600µl 10X Human CD300a Detector Antibody 1 x 600µl 1 x 600µl 10 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml 1 x 200ml 384 well CaptSure™ microplates 0 x 0 unit 1 unit 0 x 0 unit Antibody Diluent 5BI 1 x 6ml 1 x 6ml 10 x 6ml Human CD300a Lyophilized Recombinant Protein 2 vials 2 vials 20 vials Plate Seal 1 unit 1 unit 10 units Sample Diluent NS (ab193972) 0 x 0ml 1 x 50ml 1 x 50ml Sample Diluent NS (ab193972) 1 x 12ml 0 x 0ml 0 x 0ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 0 x 0 unit 10 units Stop Solution 1 x 12ml 2 x 12ml 1 x 120ml TMB Development Solution 1 x 12ml 2 x 12ml 1 x 120ml -
研究领域
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功能
Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. -
组织特异性
Expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes. -
序列相似性
Belongs to the CD300 family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
Phosphorylated on tyrosine. -
细胞定位
Cell membrane. - Information by UniProt
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别名
- CD300 antigen-like family member A
- CD300a
- CD300a antigen
see all -
数据库链接
- Entrez Gene: 11314 Human
- Omim: 606790 Human
- SwissProt: Q9UGN4 Human
- Unigene: 9688 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317784于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Example of human CD300a standard curve in Sample Diluent NS.Example of human CD300a standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of human CD300a in serum, plasma – citrate plasma – EDTA, and plasma – heparin.Interpolated concentration of native CD300a was measured in duplicate at different Human sample concentrations. Undiluted samples are 50% serum, 50% plasma - citrate, 50% plasma - EDTA, and 50% plasma - heparin. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Interpolated concentrations of human CD300a TF-1 cell culture supernatant.Interpolated concentration of native CD300a was measured in duplicate at different Human sample concentrations. Undiluted samples are 100% TF-1 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. -
Interpolated concentrations of CD300a in normal human serum donors.Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean CD300a concentration was determined to be 2,115 pg/mL with a range of 863 - 3,518 pg/mL.
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Example of human CD300a standard curve in Sample Diluent NS in 96-well vs. 384-well plate.Example of human CD300a standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of human CD300a in human serum in 96-well vs. 384-well plates.Interpolated concentration of native CD300a was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. -
Human CD300a ELISA Kit (ab317784)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Human CD300a ELISA Kit (ab317784)To learn more about the advantages of SimpleStep ELISA® kits see here.
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Human CD300a ELISA Kit (ab317784)To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
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