JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB315318

人CD146 ELISA试剂盒

Human CD146 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human CD146 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD146 with a sensitivity of 12.476 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

CD146, MUC18, MCAM, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen

4 Images
Sandwich ELISA - Human CD146 ELISA Kit (AB315318)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD146 ELISA Kit (AB315318)

Interpolated concentrations of human CD146 in HeLa cell culture supernatant

Interpolated concentration of native CD146 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HeLa cell culture supernatant 50%.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human CD146 ELISA Kit (AB315318)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD146 ELISA Kit (AB315318)

Interpolated concentrations of human CD146 in serum, plasma (citrate), plasma (EDTA), plasma (Heparin), and CSF

Interpolated concentration of native CD146 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 400, plasma (citrate) 1 : 400, plasma (EDTA) 1 : 400, plasma (heparin) 1 : 400, and CSF 1 : 50. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human CD146 ELISA Kit (AB315318)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD146 ELISA Kit (AB315318)

Interpolated concentrations of CD146 in normal human serum donors

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD146 concentration was determined to be 304 ng/mL with a range of 228 – 486 ng/mL.

Sandwich ELISA - Human CD146 ELISA Kit (AB315318)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD146 ELISA Kit (AB315318)

Example of human CD146 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cerebral Spinal Fluid, Cell culture supernatant

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 12.476 pg/mL

范围

25 - 1600 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

style
left-column

搭配使用产品

Reagents, Controls & Accessories

AB176753

Phalloidin-iFluor 488 Reagent

Immunocytochemistry/ Immunofluorescence - Phalloidin-iFluor 488 Reagent (AB176753)

  • 5
  • 14
Assay Kits

AB246525

Human TIE2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human TIE2 ELISA Kit (AB246525)

  • 0
  • 0
Reagents, Controls & Accessories

AB156034

10X RIPA Buffer

SDS-PAGE - 10X RIPA Buffer (AB156034)

  • 5
  • 2
Assay Kits

AB283881

Human ErbB2 / HER2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human ErbB2 / HER2 ELISA Kit (AB283881)

  • 0
  • 0
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

Human CD146 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD146 protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Cerebral Spinal Fluid, and Cell culture supernatant. Quantitate Human CD146 with 12.476 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style
left-column

精确度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.7" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.5" } ]
style
left-column

回收率

[ { "sample": "Serum", "range": "89 - 100 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "79 - 94 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "105 - 117 %", "average": "= 111" }, { "sample": "Heparin Plasma", "range": "94 - 104 %", "average": "= 99" }, { "sample": "Cerebral Spinal Fluid", "range": "76 - 98 %", "average": "= 88" }, { "sample": "Cell culture supernatant", "range": "93 - 110 %", "average": "= 103" } ]
style
left-column

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315318-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB315318-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315318-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB315318-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB315318-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315318-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD146 Capture Antibody", "number":"AB315318-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD146 Lyophilized Recombinant Protein", "number":"AB315318-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB315318-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD146 Detector Antibody", "number":"AB315318-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315318-CMP09", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD146 also known as MCAM or MUC18 is a cell adhesion molecule that belongs to the immunoglobulin superfamily. With a mass of approximately 113 kDa CD146 is expressed mainly on the surface of endothelial cells but it can also be found on smooth muscle cells some T-cells and in certain cancerous tissues. This protein plays a role in cell-cell adhesion contributing to the integrity and signaling functions within tissue microenvironments.
Biological function summary

CD146 influences the migration and organization of cells within the vascular system. It serves as a component of cell adhesion complexes facilitating interactions between endothelial cells and other cell types. Additionally CD146 contributes to angiogenesis the process by which new blood vessels form from existing vasculature which is critical during tissue development and repair. This function makes it an important element in maintaining normal physiological processes.

Pathways

CD146 participates in the Wnt/β-catenin and MAPK signaling pathways which are vital in cell proliferation and differentiation. Through the Wnt/β-catenin pathway CD146 interacts with other proteins like Frizzled receptors to regulate gene transcription. In the MAPK pathway it is linked with signaling cascades that involve proteins such as ERK leading to cellular responses necessary for growth and response to external stimuli.

CD146 has been implicated in melanoma progression and cardiovascular diseases. Its overexpression in melanoma associates it with increased tumor growth and metastasis often involving interactions with other adhesion molecules like integrins. In cardiovascular diseases CD146 relates to atherosclerosis as its expression on endothelial cells can promote inflammatory responses. Understanding these connections provides insights into potential therapeutic targets for treating these conditions.
style
left-column

产品实验方案

style
left-column

靶点信息

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
See full target information MCAM
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com