人CD130 ELISA试剂盒(GP130) (ab46135)
Key features and details
- Sensitivity: 15 pg/ml
- Range: 56.25 pg/ml - 1800 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人CD130 ELISA试剂盒(GP130)
参阅全部 CD130 (gp130) 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% A 8 1751pg/ml 17.61 1% B 8 264.3pg/ml 8.67 3.27% 批次间 样品 n Mean SD CV% A 16 1782pg/ml 26.64 1.49% B 16 247pg/ml 11.99 4.85% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 15 pg/ml -
范围
56.25 pg/ml - 1800 pg/ml -
回收率
97 %
特定样本回收率 样品类型 平均% 范围 Serum 97 % - % -
检测时间
1h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s CD130 (GP130) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD130 in Human serum, plasma, buffered solutions and supernatants.
A monoclonal antibody specific for CD130 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD130 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD130 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD130 present in the samples.
This kit will recognize both endogenous and recombinant Human CD130.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 2 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 200X Wash Buffer White 2 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7.5ml Biotinylated anti-CD130 Red 2 x 400µl 1 x 400µl CD130 Microplate (12 x 8 well strips) 2 units 1 unit CD130 Standard (lyophilized) Yellow 4 vials 2 vials Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml Control Silver 4 vials 2 vials HRP Diluent Red 1 x 23ml 1 x 12ml Stop Reagent Black 2 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl -
研究领域
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相关性
CD130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. -
细胞定位
Cell membrane; single-pass type I membrane protein and secreted -
别名
- CD130 antigen
- CDw130
- gp130
see all -
数据库链接
- Entrez Gene: 3572 Human
- SwissProt: P40189 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab46135于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab46135
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab46135 被引用在 1 文献中.
- Villar-Fincheira P et al. Soluble Interleukin-6 Receptor Regulates Interleukin-6-Dependent Vascular Remodeling in Long-Distance Runners. Front Physiol 12:722528 (2021). PubMed: 34707507