人C4a anaphylatoxin ELISA试剂盒
Human C4a anaphylatoxin ELISA Kit
- SimpleStep
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Human C4a anaphylatoxin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human C4a anaphylatoxin with a sensitivity of 12.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
查看别名
CO4, CPAMD2, C4A, Complement C4-A, Acidic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2
- sELISA
Supplier Data
Sandwich ELISA - Human C4a anaphylatoxin ELISA Kit (AB315320)
Interpolated concentrations of human C4a anaphylatoxin in human plasma – heparin and milk. Interpolated concentration of native C4a anaphylatoxin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human plasma – heparin 1 : 1,600 and human milk 1 : 67. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human C4a anaphylatoxin ELISA Kit (AB315320)
Interpolated concentrations of human C4a anaphylatoxin in human serum, plasma – citrate, and plasma – EDTA. Interpolated concentration of native C4a anaphylatoxin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human serum 1 : 10,000, human plasma – citrate 1 : 5,000, and human plasma – EDTA 1 : 6,400. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human C4a anaphylatoxin ELISA Kit (AB315320)
Interpolated concentrations of C4a anaphylatoxin in normal human serum donors. Serum of seven individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean C4a anaphylatoxin concentration was determined to be 4,579 ng/mL with a range of 991 – 7,804 ng/mL.
- sELISA
Lab
Sandwich ELISA - Human C4a anaphylatoxin ELISA Kit (AB315320)
Example of human C4a anaphylatoxin standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human C4a anaphylatoxin ELISA Kit (AB315320)
Representative image of Human C4a anaphylatoxin ELISA Kit (ab315320)
Components shown from left to right :
- Sample diluent NS (2 bottles)
- 10X Human C4a anaphylatoxin Detector Antibody
- Human C4a anaphylatoxin Lyophilised Purified Protein (2 vials)
- Human C4a anaphylatoxin Capture Antibody (Lyophilised)
- Stop Solution
- TMB solution
- 10X Wash Buffer PT (ab206977)
- Antibody Diluent 5BI
SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.
Plate Seals - shown underneath bottles / vials.
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit
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产品详情
Human C4a anaphylatoxin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of C4a anaphylatoxin protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, and Milk. Quantitate Human C4a anaphylatoxin with 4.245 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Complement C4a acts as an anaphylatoxin participating in the complex immune system response. Anaphylatoxins like C4a C3a and C5a contribute to the inflammatory process by inducing smooth muscle contraction increasing vascular permeability and triggering histamine release from mast cells and basophils. C4a complements the activities of other complement components playing an important role in modulating inflammation and defense mechanisms in the body.
Pathways
The activation of C4a is an important step within the classical and lectin pathways of complement activation. These pathways involve the recognition and elimination of pathogens and unwanted cells. C4a along with its related proteins C4b and components C2 and C3 facilitates the formation of C3 convertase which amplifies the response and further clears pathogens. C4a also interacts with complement inhibitors which regulate these pathways to prevent excessive tissue damage during inflammation.
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