人c-Kit ELISA试剂盒(CD117) (ab45924)
Key features and details
- Sensitivity: 0.095 ng/ml
- Range: 0.312 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人c-Kit ELISA试剂盒(CD117)
参阅全部 c-Kit 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Sample A 8 5.629ng/ml 0.34 6% Sample B 8 1.231ng/ml 0.06 4.8% 批次间 样品 n Mean SD CV% Sample A 8 5.44ng/ml 0.43 7.9% Sample B 8 1.314ng/ml 0.1 7.6% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 0.095 ng/ml -
范围
0.312 ng/ml - 10 ng/ml -
检测时间
1h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s c-Kit Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of c-Kit in Human serum, plasma, buffered solutions or supernatants.
A monoclonal antibody specific for c-Kit has been coated onto the wells of the microtiter strips provided. Samples, including standards of known c-Kit concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for c-Kit are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of c-Kit present in the samples.
This kit will recognize both endogenous and recombinant Human c-Kit.
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说明
The standard is affinity purified.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated anti c-Kit 1 x 0.4ml 2 x 0.4ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml c-Kit Control Silver 2 vials 4 vials c-Kit Microplate (12 x 8 well strips) 1 unit 2 units c-Kit Standard (Lyophilized) Yellow 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml Plastic Plate Covers 1 x 2 units 1 x 2 units Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究领域
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相关性
KIT encodes the human homolog of the proto-oncogene c-kit. c-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. -
细胞定位
Cell Membrane; single-pass type I membrane protein -
别名
- CD117
- Mast/stem cell growth factor receptor precursor
- PBT
see all -
数据库链接
- Entrez Gene: 3815 Human
- SwissProt: P10721 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab45924于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab45924 被引用在 1 文献中.
- Wang X et al. miR-18a-5p derived from mesenchymal stem cells-extracellular vesicles inhibits ovarian cancer cell proliferation, migration, invasion, and chemotherapy resistance. J Transl Med 20:258 (2022). PubMed: 35672774