人BMP2 ELISA试剂盒(ab119581)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 31.2 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人BMP2 ELISA试剂盒
参阅全部 BMP2 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 1 16 55pg/ml 4.29 = 7.8% 2 16 259pg/ml 13.25 = 5.3% 3 16 1005ng/ml 70.35 = 7% 批次间 样品 n Mean SD CV% 1 24 59pg/ml 4.66 = 7.9% 2 24 255pg/ml 15.55 = 6.1% 3 24 1061pg/ml 83.81 = 7.9% -
样品类型
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts -
检测类型
Sandwich (quantitative) -
灵敏度
< 2 pg/ml -
范围
31.2 pg/ml - 2000 pg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Human BMP2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human BMP2 in bone tissue, cell culture supernatants and serum.
A BMP2 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection monoclonal antibody from mouse, specific for BMP2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human BMP2 amount of sample captured in plate.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-Human BMP2 antibody Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti- human BMP2 antibody 1 x 100µl 1 x 100µl Lyophilized recombinant human BMP2 standard 2 x 10ng 2 x 10ng Plate Seal 1 x 4 units 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
研究领域
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功能
Induces cartilage and bone formation. -
组织特异性
Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine. -
序列相似性
Belongs to the TGF-beta family. -
细胞定位
Secreted. - Information by UniProt
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别名
- BDA2
- BMP-2
- BMP-2A
see all -
数据库链接
- Entrez Gene: 650 Human
- Omim: 112261 Human
- SwissProt: P12643 Human
- Unigene: 73853 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119581于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab119581.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab119581 被引用在 11 文献中.
- E L et al. Microenvironment Influences on Human Umbilical Cord Mesenchymal Stem Cell-Based Bone Regeneration. Stem Cells Int 2021:4465022 (2021). PubMed: 34447439
- Chen Z et al. Fabricating a novel HLC-hBMP2 fusion protein for the treatment of bone defects. J Control Release 329:270-285 (2021). PubMed: 33278483
- Lee SS et al. A Biomimetic Macroporous Hybrid Scaffold with Sustained Drug Delivery for Enhanced Bone Regeneration. Biomacromolecules 22:2460-2471 (2021). PubMed: 33971092
- Xie S et al. Recombinant human bone morphogenetic protein 2 and 7 inhibit the degeneration of intervertebral discs by blocking the Puma-dependent apoptotic signaling. Int J Biol Sci 17:2367-2379 (2021). PubMed: 34239363
- Liu F et al. USF2 enhances the osteogenic differentiation of PDLCs by promoting ATF4 transcriptional activities. J Periodontal Res 55:68-76 (2020). PubMed: 31448831